大白菜开花相关基因CO的BAC克隆筛选及分析  

Screening and Analysis of BAC Clones Containing Flowering Time Gene CO in Brassica campestrisL.ssp.pekinensisOlsson

作　　者：马爱茹[1] 冯大领[1] 刘立平[1] 轩淑欣[1] 王彦华[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071000

出　　处：《华北农学报》2012年第6期20-23,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金(31171964);河北省自然科学基金(C2010000677;C2010000738);高等学校博士学科点专项科研基金(20101302110001)

摘　　要：根据已经公布的大白菜CO基因(Bra008669)序列设计特异引物,采用三维PCR法筛选大白菜BAC文库,获得了3个含有CO的BAC克隆。对筛选到的3个BAC克隆进行PCR扩增,将克隆测序结果与大白菜CO基因进行序列比对,结果表明,相似性达到99.73%,且N端有保守B-BOX结构,C端有保守的CCT结构域,证明所筛选的3个BAC克隆是含有大白菜CO基因的克隆。The specific primers designed according to the published Chinese cabbage CO gene（Bra008669） were used to screen the Chinese cabbage BAC library through three steps of PCR,and 3 clones containing CO gene were obtained.The 3 obtained clones were amplificated and the sequence compared with Chinese cabbage CO gene（Bra008669）,the result showed that the amplificated fragments had 99.73% of homology with the CO gene（Bra008669） and included N-terminal and C-terminal conservated domains.

关 键 词：大白菜 开花相关基因 CO基因 BAC克隆 

分 类 号：Q78[生物学—分子生物学]