亲水色谱法分析白坚木皮醇的研究  

Determination of Quebrachitol Using Hydrophilic Interaction Chromatography

作　　者：薛敏[1] 吕雉[1] 伍英 姜士宽 陈婧[1] 孟子晖[1] 

机构地区：[1]北京理工大学化工与环境学院,北京100081 [2]云南省热带作物研究所,景洪666100

出　　处：《天然产物研究与开发》2012年第12期1715-1718,共4页Natural Product Research and Development

基　　金：国家科技支撑计划(2011BAD;30B01);北京理工大学研究生创新平台资助

摘　　要：白坚木皮醇是天然橡胶制胶乳液中存在的一种具有极高药用价值的天然化合物,多存在于制胶废水中。由于其极性高且溶于水,在常用的反相液相色谱柱中保留值较低,分析和分离都较困难。本研究选用SHI-SEIDO PC HILIC色谱柱建立了亲水色谱法(HILIC)测定白坚木皮醇的方法。以乙腈-水(V/V,60/40)为流动相,二极管阵列(DAD)为检测器时,检测波长为190 nm,白坚木皮醇的保留因子为1.07,线性范围为0.05～4mg/mL,检测限0.01 mg/mL。Abstraet：L-Quebrachitol,with significant medical value, was recently found in rubber latex. Due to its hydroxyl radi- cals ,the determination of this compound becomes a difficult issue using traditional reverse-phase liquid chromatography （RPLC） with C-18 column. Therefore,SHISEIDO PC HILIC HPLC with DAD was performed in this study and the mo- bile phase was fixed as aeetonitrile-water （ V/V ,6/4）. The range of linearity and limit of detection with the DAD detec- tion wavelength 190 nm were 0. 05-4 mg/mL and 0.01 mg/mL. Meanwhile, the retention factor of L-quebraehitol was 1.07 in DAD detection mode.

关 键 词：白坚木皮醇 亲水色谱 高效液相法 制胶废水 

分 类 号：R917[医药卫生—药物分析学]