黄酮类化合物清除羟自由基(HO·)功能及其构效关系研究  

Function-structure Association of Flavonoid Phytochemicals on Scavenging Hydroxyl Radical(HO·)

作　　者：郭春梅[1] 刘淑清[2] 郭一萌[2] 鱼红闪[3] 孙明忠[1] 

机构地区：[1]大连医科大学生物技术系 [2]大连医科大学生物化学与分子生物学教研室,大连116044 [3]大连工业大学生物工程学院,大连116034

出　　处：《天然产物研究与开发》2012年第12期1724-1728,共5页Natural Product Research and Development

基　　金：国家自然科学基金(81171957);辽宁省教育厅科研项目(2008S077;LS2010050);辽宁省自然科学基金(20082158)

摘　　要：本文采用体外实验的方法,系统地研究了八种黄酮类植物化学物槲皮素、异槲皮素、芦丁、橙皮素、橙皮苷、柚皮素、柚皮苷和淫羊藿苷对羟自由基(HO.)的清除作用及构效关系。结果表明,八种黄酮类化合物中,只有槲皮素、芦丁和异槲皮素对HO·有明显的清除作用,在浓度为260μM时其对HO·清除率分别为30.69%±0.06、13.70%±0.04和10.23%±0.03。构效比较结果表明:黄酮类化合物C环C3位羟基和B环3’-4’邻位二羟基是其清除HO.的重要功能基团。本研究为选择有效的能清除HO·的膳食黄酮类化合物用于预防和治疗相关疾病提供了一定实验基础和理论依据。The function-structure association of eight flavonoids including phytochemicals quercetin, isoquercitrin, rutin, hesperetin, hesperidin, naringenin, naringin and icariin on scavenging hydroxyl radical （HO ·） were systemically inves- tigated in vitro. The results indicated that among the eight flavonoids only quercetin, rutin and isoquereitrin showed ap- parent scavenging HO ·activity at the concentration of 260 μM with the scavenging percentages of 30.69%± 0. 06, 13. 70%±0.04 and 10.23% ±0.03 ,respectively. The function-structure relationship study indicated that the C ring 3- OH group and B ring 3＇ -4＇ orthodihydroxy group of flavonoids were important for their scavenging HO · function. This study might provide certain insights for selecting effective and favorable dietary flavonoids in the prevention and treat- ment of related diseases.

关 键 词：黄酮类化合物 抗自由基 结构 功能 

分 类 号：Q956[生物学—动物学]