芳香植物广陈皮和山奈中有翅桃蚜驱避物质的化学鉴定  

Chemical Analysis of Active Volatile Components in Two Aroma Plants, Citrus chachiensis and Kaempferia galanga, Responsible for Repelling Alate Myzus persicae(Sulzer)

作　　者：李为争[1] 庄丽[1] 付国需[1] 杨雷[1] 李慧玲[1] 原国辉[1] 

机构地区：[1]河南农业大学植物保护学院,郑州450002

出　　处：《天然产物研究与开发》2012年第12期1821-1827,1796,共8页Natural Product Research and Development

基　　金：国家公益性行业(农业)专项资助项目(201203036);河南省科技攻关重点项目(082102150003)

摘　　要：芳香植物广陈皮和山奈的挥发物对有翅桃蚜具有较强的驱避作用,本试验采用提取、浓缩、柱层析和GC-MS等技术鉴定了其中的活性成分。生物测定结果表明,索氏提取法能够保留原始材料的驱避效果,且广陈皮和山奈的索氏提取物存在加成效应或微弱的拮抗效应。分别采用梯度混合的石油醚-乙醚混合淋洗剂对两种材料的索氏提取物进行柱层析分离,发现广陈皮仅3∶7体积比的石油醚-乙醚洗脱液有活性;山奈层析物中驱避作用最强的是石油醚-乙醚等体积混合淋洗的馏分,驱避百分率达到45.56%,另外7∶3和2∶8体积比的石油醚-乙醚淋洗物也有相对较弱的驱避活性。GC-MS分析表明,广陈皮活性馏分的主要挥发物成分为D-柠檬烯(39.07%),山奈活性馏分的主要挥发物成分为反-对甲氧基肉桂酸乙酯(70.22%)和顺-对甲氧基肉桂酸乙酯(19.55%),两类化合物对桃蚜具有较强驱避活性,且两者之间仍表现为加成效应。Two aroma plants,Citrus chachienis kneel and Kaempferia galanga root,were previously shown to repel alate Myzus persicae （Sulzer）. In this paper, the active components were identified via Soxlet extraction, condensation, column chromatography, and gas chromatography-mass spectrometry （GC-MS）. Both Soxlet extracts retained the repellent effec- tiveness of their corresponding origins, but bioassay of the sequential binary combinations indicates that C. chachiensis extract and K. galangal extract exhibited additive or weak antagonistic interaction. The Soxlet extracts were subjected to bioassay-guided column chromatography using petroleum ether and diethyl ether as solvent system, totally 11 fractions were collected from each extract. Among C. chachiensis fractions, only one fraction （ rinsed with 3： 7 volume ratio of pe- troleum ether and diethyl ether） showed extremely significant repellence;amung K. galangal fractions,the one rinsed by equal volume of petroleum ether and diethyl ether showied the strongest repellence, whose repellent percentage reached to 45.56% ,and the 7：3 and 2：8 fractions also showed relatively low activity. GC-MS analysis indicates that the major volatile of C. chachiensis active fraction was D-limonene （39.07%） , and that of K. galangal were trans ethyl p-methoxy cinnamate （70.22%） and cis ethyl p-methoxy cinnamate （19.55%） ,further bioassay confirmed the activities of D-li- monene and trans ethyl p-methoxy cinnamate, and the two compounds also exhibited additive interaction as their origins.

关 键 词：桃蚜 芳香植物 广陈皮 山奈 驱避成分 

分 类 号：Q968.1[生物学—昆虫学] S436.3[农业科学—农业昆虫与害虫防治]