导入普通野生稻DNA的水稻变异种质的耐盐性鉴定  

Salt Tolerance of Rice Variation Germplasm with Transformed Common Wild Rice (Oryza rufipogon) DNA

作　　者：吕学莲[1] 白海波[1] 李树华[1] 蔡正云[2] 高晓原[1] 

机构地区：[1]宁夏农业生物技术重点实验室,银川750002 [2]宁夏大学,银川750021

出　　处：《中国农学通报》2012年第36期41-45,共5页Chinese Agricultural Science Bulletin

基　　金：宁夏农林科学院自主研发项目"利用DNA导入技术培育高产抗逆水稻新品种";国家"973"计划项目子课题"鉴定由调控网络重要节点构成的调控单元;并进行耐盐碱作物分子育种设计"(2012CB114204)

摘　　要：旨在研究导入普通野生稻DNA的水稻后代的耐盐性,从中筛选出耐盐性较强的材料,为水稻耐盐育种提供种质资源。以普通野生稻DNA导入到宁夏栽培水稻‘宁粳23号’中获得的10份变异后代为材料,研究各材料在盐胁迫下的发芽特性和幼苗生长情况。结果表明在170mmol/LNaCl处理下,材料D42、D5-54、D5-27、D-10、D102、DJ18和D68的发芽势和发芽率均较高,相对盐害率较低,在盐浓度为120mmol/L时,材料D5-27、DJ18、D5-54和D-10的耐盐性为强,地上和地下鲜、干重的盐处理与非盐处理的比值较高,材料D5-27、DJ18、D5-54和D-10的可溶性糖积累量高于其受体。材料D42、D5-54、D5-27、D-10、D102、DJ18和D68的芽期耐盐性较强,材料D5-27、DJ18、D5-54和D-10属于苗期较耐盐的材料。The research aimed to study salt tolerance of rice variation germplasm with transformed common wild rice DNA, and screen out the materials with high salt tolerance, so as to provide germplasm for salt-tolerant rice breeding. Ten rice variation germplasms of ＇Ningjing 23＇ were used to study germination and seedling growth. The results showed that, when treated with 170 mmol/L NaC1, the germination level and germination rate of materials D42, D5-54, D5-27, D-10, D102, DJ18 and D68 were high, and its relative salt harm rate were low. With the solution containing 120 mmol/L NaC1, the salt tolerance of D5-27, DJ18, D5-54 and D-10 were strong, and the ratio of salt treatment and non-salt treatment of ground and underground, fresh and dry weights were high. The increased soluble sugar content of materials D5-27, DJ18, D5-54 and D-10 were higher than its receptor. Materials D42, D5-54, D5-27, D-10, D102, DJ18 and D68 had high salt tolerance in germinating stage, and materials D5-27, DJ18, D5-54 and D-10 had high salt tolerance in seedling stage.

关 键 词：野生稻 导入后代 耐盐性 鉴定 

分 类 号：S511[农业科学—作物学]