桑花叶型萎缩病病原的检测与序列分析  

The Detection and Sequence Analysis of Pathogen from Mulberry Mosaic Dwarf Disease

作　　者：孔卫青[1] 杨金宏[1] 

机构地区：[1]安康学院/陕西省蚕桑重点实验室,陕西安康725000

出　　处：《中国农学通报》2012年第36期237-240,共4页Chinese Agricultural Science Bulletin

基　　金：陕西省科技厅农业攻关项目"抗花叶型萎缩病转基因桑树选育"(2011K01-40);陕西省教育厅重点实验室项目"核酶在桑树类病毒病害防治中的应用"(11JS001)

摘　　要：旨在获得安康地区桑花叶萎缩病病原序列,为桑花叶萎缩病的防治提供更多的信息。用RT-PCR技术对安康地区染花叶型萎缩病病株的病原进行了分离,克隆测序并在Mfold网站预测其二级结构。该病的病原为桑花叶萎缩类病毒MMDVd-201110151(GenBank登录号:JQ809700),核苷酸长度357bp,GC含量50.7%,219bp后38个碱基与樱桃小圆形类病毒样核糖核酸具有约85%的一致性。Mfold网站预测二级结构有58.3%的碱基配对,两端为分枝状结构。成功分离到安康地区桑花叶型萎缩病病原。The aims were to obtain the pathogenic sequence of mulberry mosaic dwarf disease in Ankang area, and provide more information on the control of the disease. The pathogen of mulberry mosaic dwarf disease in Ankang area was isolated by RT-PCR, and the secondary structure was predicted in Mfold web. The length of the pathogen, MMDVd-201110151 （GenBank accession number： JQ809700）, was 357 bp, and the GC percent was 50.7% in it. The 38 bp behind 219 bp had 85% identity with cscRNAl.150. The secondary structure of MMDVd-201110151 was predicted in Mfold web, 58.3% pairs in the secondary structure, and the two ends in it were both branched. The pathogen of mulberry mosaic dwarf disease in Ankang area was isolated successfully.

关 键 词：桑花叶型萎缩病 类病毒 序列分析 二级结构 

分 类 号：S888.712[农业科学—特种经济动物饲养]