香根草转AtCBF3基因体系的建立及对其抗寒性的影响  

Establishment of Agrobacterium-mediated AtCBF3 Transformation and its Effect on Cold Tolerance in Vetiver (Vetiveria zizanioides)

作　　者：代利平[1] 吕冰[1] 唐瑭[2] 郦汀汶[1] 谢虹[1] 梁建生[1] 

机构地区：[1]扬州大学生物科学与技术学院 [2]江苏省作物遗传生理国家重点实验室,江苏扬州225009

出　　处：《玉林师范学院学报》2012年第5期2-6,共5页Journal of Yulin Normal University

基　　金：江苏省环洪泽湖生态农业生物技术重点实验室开放基金资助项目(HZHL0805)~~

摘　　要：温度是影响香根草在温、寒带推广应用的主要限制因子.为提高香根草的耐寒性,利用分子遗传学手段将拟南芥基因组中的转录激活因子基因AtCBF3导入香根草中,实现种质改良.本研究从野生型拟南芥哥伦比亚Columbia植株中克隆出AtCBF3,经酶切连接构建了表达载体pCAMBIA1301/AtCBF3.再通过农杆菌介导法导入外源基因后,在含抗生素培养基上筛选获得抗性植株,后经PCR和越冬试验后证实获得了抗寒性增强的转基因香根草植株.Temperature is the main restricted factor for distribution and application of vetiver （Vetiveria zizanioides） in Temperate and Frigid Zone. To improve its chilling resistance, we established an Agrobacterium- mediated gen transfer system, in which a gene encoding transcriptional activator CBF3 extracted from Arabidopsis was transferred into the vetiver calli. The research results showed that the transgenic vetiver seedlings which overexpressed CBF3 were more tolerant to low temt3erature stress.

关 键 词：遗传转化 AtCBF3 抗寒性 香根草 

分 类 号：Q785[生物学—分子生物学]