黄芪多糖抗去势雌鼠骨质疏松的实验研究  

Experimental Study on Anti-Osteoporosis Effect of Astragalts Polysaccharides on Ovariectomized Female Rats

作　　者：张宏波[1] 曹文波[1] 崔文璟[2] 张振华[1] 莫赛军[1] 

机构地区：[1]郑州大学基础医学院,河南郑州450001 [2]江南大学生物工程学院,江苏无锡450008

出　　处：《玉林师范学院学报》2012年第5期51-55,共5页Journal of Yulin Normal University

基　　金：江南大学工业生物技术教育部重点实验室开放课题(KLIB-KF201107)

摘　　要：目的:评价黄芪多糖预防大鼠卵巢切除后骨质疏松的药效学.方法:取8月龄雌性SD大鼠44只,随机分为4组,每组11只.黄芪多糖组(APS组)大鼠切除双侧卵巢后,黄芪多糖灌胃12周,与单纯卵巢切除组(OVX组)、假手术组(SHAM组)及尼尔雌醇灌胃组(E3组)比较.采用血尿生化检测、骨干/湿重、骨生物力学、骨密度测量和骨形态计量学等方法对黄芪多糖的药效学进行综合评价.结果:与SHAM组比较,OVX组骨干重、骨灰重、骨钙含量和骨密度、抗弯力、骨小梁体积密度及宽度均明显降低,而成骨细胞指数和破骨细胞指数、尿羟脯氨酸则明显增高.黄芪多糖及E3能使上述指标显著改善,接近正常水平.结论:黄芪多糖具有明显抗去势雌鼠骨质疏松作用.Purpose： To evaluate the anti-osteoporosis effect of astragalts polysaccharides in ovariectomized female rats. Methods： 44 female SD rats aged 8 months were divided randomly into 4 groups： the SHAM group （subjected to sham operation）; the OVX group （subjected to bilateral ovariectomy）; the E3 group （subjected to bilateral ovariectomy and treated after surgery with nilestriol lmg/kg BW by gavage once a week for 12 weeks）, and the APS group （subjected to bilateral ovariectomy and treated after surgery with astragalts polysaccharides lg/kg BW by garage once a day for 12 weeks）. All the animals were sacrified 12 weeks after the operation. The blood and urine samples were collected for determination of biochemical indices. The humeri and tibiae were prepared for determination of net weight, ash weight, calcium and phosphorus content, bone density, as well as bone histopathology and histomorphometry. The left femora were processed for bone anti-curve power. Results： Compared with the SHAM group, the net weight, ash weight, calcium content, density and bending resistance of bones, the trabecular bone volume and thickness were all significantly reduced in the OVX group, while its osteoblast index, osteoclast index and urine hydroxyproline content were obviously increased. In the E3 group and the astragalts polysaccharides group, these indices were close to normal levels. Conclusion： Astragalts polysaccharides can effectively prevent osteoporosis in ovariectomized rats.

关 键 词：黄芪多糖 卵巢切除 骨质疏松 

分 类 号：R285.5[医药卫生—中药学] R681[医药卫生—中医学]