致仔猪腹泻大肠杆菌的耐药性与质粒谱分析  

Analysis on Drug Resistance and Plasmid Profile of Escherichia Coli in Piglets

作　　者：刘玉芹[1] 陈翠珍[1] 杨彩然[1] 贾青辉[1] 董淑珍[1] 李蓉[1] 郑新羽[1] 房海[1] 

机构地区：[1]河北科技师范学院动物科技学院河北省预防兽医学重点实验室,河北秦皇岛066600

出　　处：《河北科技师范学院学报》2012年第4期47-50,共4页Journal of Hebei Normal University of Science & Technology

基　　金：河北省科技支撑计划项目(项目编号:09220402D);河北省自然科学基金项目(项目编号:C2009000869);河北省教育厅项目(项目编号:Z2009114)

摘　　要：对河北省不同地区发病猪场分离的已经过系统鉴定的12株猪源大肠杆菌,采用K-B纸片法进行了15种抗生素的耐药性检测,并提取质粒,对耐药谱和质粒谱型进行了比较分析。结果显示,分离菌株对氨苄西林的耐药率达到了100%,对阿莫西林的耐药率为91.7%,对四环素和复方新诺明的耐药率介于50%～70%。分离菌株存在多重耐药性,以耐4种、6种和7种药物为主,占总数的75%。对分离菌株耐药谱与质粒谱的分析表明,大肠杆菌的质粒携带与细菌的耐药性之间并没有明显的对应关系。In this experiment,the antimicrobial susceptibilities to 15 antibiotics of 12 pathogenic Escherichia co- li from piglets of different cities and counties in Hebei province were determined by Kirby-Bauer method. The plasmid was extracted and 12 drug-resistant strains were analyzed for molecular typing and plasmid homology cluster. The results showed that： 100 resistance rate against ampicillin and 91.7 against Amoxicillin. The re- sistance rate against tetracyclineand trimethoprim and sulphame-thoxazole were 50%- 70%. Multi-drug resist- ance test showed that 75% E. coli strains were 4,6 and 7 kinds of antibiotics resistance. The analysis of com- parative resistance spectra and plasmid profile showed that there was no direct relationship between the drug resistance and the number and size of plasmids.

关 键 词：大肠杆菌 耐药性 质粒谱 

分 类 号：S852.612[农业科学—基础兽医学]