鹅肌肉组织中MC4R mRNA表达量与屠宰性能的相关性分析  

Analysis of the Correlation between Slaughter Performance and Tissue MC4R mRNA of Goose Muscle

作　　者：隋玉健[1] 孙永峰[1] 林海涛[1] 朱海洋[1] 张磊 吴伟[1] 高光[1] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118 [2]江西省畜牧技术推广站,南昌330046

出　　处：《吉林农业大学学报》2012年第6期672-676,共5页Journal of Jilin Agricultural University

基　　金：吉林省教育厅吉林省高校优秀青年科研人才春苗培育计划项目(2012);长春市科技局农业科技推广示范计划项目(11NS12);吉林省现代农业产业技术体系建设专项资金资助项目(201231);江苏省创新团队项目(2012)

摘　　要：采用单因子试验设计,选用160只体质量相近的0日龄吉林白鹅和农安籽鹅为试验动物,每个品种随机分为4组,饲喂以NRC标准为基础配制日粮,分0~4周龄和5~8周龄2个阶段饲养。通过屠宰试验和RT-PCR方法对鹅的MC4RmRNA在胸肌和腿肌组织中的表达量进行了检测,进而比较不同遗传基础鹅MC4RmRNA表达的差异。结果表明：MC4RmRNA表达量与屠宰性状有一定的相关性,吉林白鹅胸肌、腿肌MC4RmRNA表达量高于农安籽鹅,吉林白鹅活体重、胸肌重、腿肌重、日增重均高于农安籽鹅。Single-factor experiment was designed,and 160 natal Jilin white geese and Nongan geese of the similar body weight were chosen as experimental animal. The geese of either variety were then divided into 4 treatment groups randomly and NRC-based dietary was fed to them according to their two developmental stages,0 to 4 weeks old,and 5 to 8 weeks old.By slaughter experiments and RT-PCR（reverse transcription polymerase chain reaction） technique,the expressions of MC4R（melanocortin receptor-4）and its receptor in liver,breast and leg muscles during different developmental stages were detected to further study the influence of different genetic basis on levels of MC4R mRNA and its receptor.The results show that the meat performance of different genetic basis and the expression level of MC4R mRNA in muscle show a correlation of some extent.The expression level of MC4R mRNA in leg or in breast muscle of Jilin white geese is higher than that in Nongan geese.Live weight,leg muscle weight,breast muscle weight,daily gain weight of Jilin white geese are higher than those of Nongan geese.

关 键 词：鹅 MC4R mRNA 实时荧光定量 屠宰性状 

分 类 号：S835[农业科学—畜牧学] Q786[农业科学—畜牧兽医]