阿卡斑(赤羽病)病毒的分离与初步鉴定  

Isolation and Preliminary Identification of Akabane Virus

作　　者：李其平[1] 周立桥[1] 姚龙涛 蔡玉书 陈向前[1] 卫龙兴 李昌琳[1] 王新 丁江[1] 李金萍 李业红 张雪莲 

机构地区：[1]农业部动物检疫所,青岛266032 [2]上海市奉贤县畜牧兽医站 [3]河北张家口农业高等专科学校动物医学系

出　　处：《中国动物检疫》2000年第7期27-29,共3页China Animal Health Inspection

摘　　要：１９９８年７月－８月从上海地区采集的蚊、蠓等标本中分离到１２株病毒，其中有３株电镜观察病毒近似球形，有囊膜，病毒对乙醚、氯仿和胰蛋白酶敏感，对酸、热不敏感，抵抗５－氟脱氧尿苷。经血清－细胞和动物中和试验鉴定，该三株病毒均能被ＡＫＶ病毒高效价免疫血清中和，其余９株病毒未被ＡＫＶ高价免疫血清中和，经初步鉴定证实上述三株病毒为阿卡斑病毒（Ａｋａｂａｎｅ ｖｉｒｕｓ， ＡＫＶ）。阿卡斑病毒在我国的分离尚属首次报道，具有重要的动物流行病学意义。isolates were Obtained from 30 000 mosquitoes collected in Fengxian Country, Shanghai during July and AUgUst 1998 and 3 of them were of spheriform with envelop Under EM. They were sensitive to ether, chloroform and sin, not sensitive to acid and heat treatment and resistant to floxuridine. The three isolates induced CPE in SVP,Vero and BHK-21 cells and were neutralized by AKV hyperimmune serum, while the other nine isolates were not. It was preliminarily demonstrated that the three isolates were Akabane virus. It is the first report on the isolation and identification of the virus in China, having important epizootiological significance.

关 键 词：阿卡斑病毒 蚊 上海 

分 类 号：S852.65[农业科学—基础兽医学]