去细胞犬喉支架的体内埋植实验  

Study on decellularized laryngeal scaffold in dogs

作　　者：李万里[1] 罗家胜[1] 刘飞[1] 马瑞娜[1] 陈志峰[1] 崔鹏程[1] 

机构地区：[1]第四军医大学唐都医院耳鼻咽喉头颈外科,西安710038

出　　处：《中华耳鼻咽喉头颈外科杂志》2013年第1期54-60,共7页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：国家自然科学基金（81070776）

摘　　要：目的探讨去细胞犬喉支架的性质及其在同种异体犬胸肌内存活的可行性。方法实验组18个供体喉应用十二烷基磺酸钠行去细胞处理，3个用于组织学等检测，15个埋在受体犬胸肌内；对照组供体喉不去细胞，余实验步骤同实验组。两受体组于2周、1个月、2个月各处死5只犬，行大体观察、组织学检查及台盼蓝染色。结果对照组的新鲜喉埋植后缩小甚至消失，与实验组比较采用最小显著差异t检验差异有统计学意义（P〈0．05）。实验组去细胞喉支架埋植2周时显微镜观察支架周围仅有少量中性粒细胞及淋巴细胞浸润，1个月后支架周围疏松结缔组织开始形成，2个月时结缔组织明显增厚，血管数量明显增多。对照组埋植2周时大量淋巴细胞及中性粒细胞浸润，1个月时软骨膜被破坏，2个月时软骨细胞消失。埋植前实验组与对照组软骨细胞平均（x±s，下同）存活率分别为86．8％±3．2％和88．6％±3．1％，采用卡方检验差异无统计学意义（X2=0．19，P〉0．05）；埋植2周和1个月后实验组软骨细胞存活率下降不多而对照组逐渐下降明显，差异均有统计学意义（X2分别为5．52和20．55，P值分别〈0．05和〈0．01）；2个月时实验组存活率为65．8％±2．6％，而对照组软骨细胞全部消失。结论去细胞方法可得到低免疫原性的去细胞喉支架，该支架能够在胸肌包裹下存活，并建立血供，有可能用做全喉重建的生物支架材料。Objective To explore the survivorship and character of decellularized laryngeal scaffold in pectoralis major muscle flap in canine. Methods Eighteen donor larynxes in experimental group were decellularized by perfusing sodium dodecyl sulphate. Three of them were used to detect the character of histology. The other fifteen ones were embedded in right pectoralis major muscle flap of acceptor canine. Donor larynxes in control group were not perfused. Other experimental procedure was the same as experimental group. The specimens were harvested at two weeks, one month and two months after operation, respectively. Macroscopic view, histological examination and trypan blue staining were performed in the experimental group and control group. Results The size of the specimens decreased remarkably into disappearance in control group, there was statistical significance betwwn the experimental group and the control group （ which used least significant difference t test P 〈 0.05 ）. There was only little neutrophils and lymphoeytes infiltrating around the laryngeal scaffold at 2 weeks in the experimental group. One month after operation, loose connective tissue begin to form aound the laryngeal scaffold. After two months of transplation, the connective tissue became thicker and the number of blood vessels increased than before. There was a large number of lymphocytes and neutrophil infiltration around the laryngeal specimens in the control group at 2nd week. The perichondrium in the control group was damaged at one month post operation. The cartilage cells could not be detected two months after surgery. The survival rate of cartilage cell between experimental group （86. 8% ± 3.2% ） and the control group （88.6% ± 3.1% ） did not show statistical significance before implantation （ X2 = 0. 19, P 〉 0. 05 ）. The survival rate of cartilage cell decreased insignificantly in experimental group while the survival rate declined obviously in the control group at two weeks and one month after operation, the difference

关 键 词：喉 支架 植入物 实验性 软骨细胞 器官移植 狗 

分 类 号：R767.91[医药卫生—耳鼻咽喉科]