吡格列酮对高脂饲养诱导胰岛素抵抗大鼠胰腺组织PTP-1B表达的影响  

Influences of pioglitazone on the appearance of protein tyrosine phosphatase 1B in the pancreas of high fat diet induced insulin resistance rats

作　　者：于苏国[1] 王令令[2] 孙吉花[3] 王迎雪[1] 赵慧[1] 

机构地区：[1]滨州医学院附属医院内分泌科,山东滨州256603 [2]滨州市人民医院干部保健一科,山东滨州256603 [3]滨州医学院附属医院院感管理科,山东滨州256603

出　　处：《山东大学学报（医学版）》2013年第1期17-21,共5页Journal of Shandong University:Health Sciences

摘　　要：目的观察吡格列酮对胰岛素抵抗SD大鼠胰腺组织中蛋白酪氨酸磷酸酶1B(PTP-1B)表达的影响。方法选取SD大鼠60只,随机分为对照组(NC组,n=20),喂养普通饲料;高脂组(HF组,n=40)同时分为高脂对照组(HFN组,n=20)和高脂吡格列酮组(HFP组,n=20),喂养高脂饲料12周后,进行模型验证。HFP组给予吡格列酮30 mg/(kg.d)灌胃2周,NC组和HFN组给予相同体积的生理盐水灌胃,应用免疫组化和western blot技术测定大鼠胰腺组织中PTP-1B蛋白的表达。结果 HF组大鼠胰岛素敏感性较NC组大鼠明显降低,差异有统计学意义(P<0.01);HFN组、HFP组胰腺组织PTP-1B表达量较NC组明显增高(P<0.01),与HFN组相比,HFP组PTP-1B表达量明显减少(P<0.01)。结论高脂饮食诱导的肥胖大鼠产生胰岛素抵抗,同时使其胰腺中PTP-1B蛋白表达量升高。吡格列酮可以降低胰腺组织中PTP-1B的表达,改善胰岛素抵抗。Objective To observe the effect of Pioglitazone on the appearance of protein tyrosine phosphatase 1B in the pancreas of insulin resistance rats. Methods 60 SD rats were randomly divided into the control group （ NC group, n = 10） on regular diet, the high fat diet group （HFN group, n = 15） and the high fat diet group administrated with Piogli- tazone （HFP group, n = 15）. The last two groups （HF groups） were on high fat diet. After twelve weeks, verify the features of the models. The HFP group were treated with Pioglitazone [ 30 mg/（ kg . d）, intragastric administration ] daily for 2 weeks. The NC group and the HFN group received a saline intragastric administration of equal volume. The expression of PTP-IB in the pancreas of rats was detected by immunohistochemlstry and Western blot. Results The sen- sitivity of insulin was degraded in the HF group than that in the NC group （P 〈 0.01 ）. The immunohistochemistry and Western blot test showed that the expression of PTP-1B protein in the HF group was higher than that in the NC group （ P 〈 0.01 ）. The expression of PTP-1B protein in the HFP group significantly decreased compared with that in the HFNgroup （ P 〈 0.01 ）. Conclusion The obese rats induced by high fat diet show insulin resistance. At the same time, the PTP-1B level in their pancreas increase. Pioglitazone could degrade the expression of PTP-1B in the pancreas and im- prove the insulin resistance.

关 键 词：蛋白酪氨酸磷酸酶-1B 胰岛素抵抗 胰腺组织 吡格列酮 

分 类 号：R587.1[医药卫生—内分泌]