Clinicobiochemical investigations of gangrenous mastitis in does:immunological responses and oxidative stress biomarkers  

作　　者：Wael M.EL-DEEB 

机构地区：[1]Department of Clinical Studies,College of Veterinary Medicine and Animal Resources,King Faisal University [2]Department of Veterinary Medicine,Infectious Diseases and Fish Diseases,Faculty of Veterinary Medicine,Mansoura University

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2013年第1期33-39,共7页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project (No. 110031) supported by the Deanship of Scientific Research,King Faisal University,Saudi Arabia

摘　　要：A total of 50 does were used to determine selected hematological and biochemical parameters with special references to oxidative stress markers, acute phase protein profiles, and proinflammatory cytokines in healthy and gangrenous mastitis affected does. Animals were divided into two equal groups represented as clinically healthy （control） and diseased groups, respectively. The bacteriological examination of milk samples from diseased does revealed many types of bacterial infection. The isolated bacteria were Staphy/ococcus aureus （N=23/25）, Escherichia coil （N=11/25）, and Clostridium perfringens （N=4/25）. There was a significant increase in the levels of β-hydrexybutyrate, non-esterified free fatty acids, triglyceride, low density lipoprotein cholesterol （LDL-C）, aspartate aminotransferase, and alanine aminotransferase and a significant reduction in the levels of glucose, cholesterol, and high density lipoprotein cholesterol （HDL-C） in does with gangrenous mastitis compared to healthy does. Moreover, there was a significant increase in the levels of malondialdehyde and uric acid with a significant decrease in the levels of reduced glutathione, super oxide dismutase, and catalase in does with gangrenous mastitis compared to healthy does. In addition, there was a significant increase in the haptoglobin, serum amyloid A, fibrinogen, interleukin 6 （IL-6）, IL-1β, and tumor necrosis factor-a （TNF-a） in does with gangrenous mastitis compared to healthy ones. Conclusively, oxidative stress biomarkers, acute phase proteins, and proinflammatory cytokines play an essential task as biomarkers for gangrenous mastitis in does. Mastitis may be considered as one of the ketotic stressors in does after parturition.A total of 50 does were used to determine selected hematological and biochemical parameters with special references to oxidative stress markers,acute phase protein profiles,and proinflammatory cytokines in healthy and gangrenous mastitis affected does.Animals were divided into two equal groups represented as clinically healthy(control) and diseased groups,respectively.The bacteriological examination of milk samples from diseased does revealed many types of bacterial infection.The isolated bacteria were Staphylococcus aureus(N=23/25),Escherichia coli(N=11/25),and Clostridium perfringens(N=4/25).There was a significant increase in the levels of β-hydroxybutyrate,non-esterified free fatty acids,triglyceride,low density lipoprotein cholesterol(LDL-C),aspartate aminotransferase,and alanine aminotransferase and a significant reduction in the levels of glucose,cholesterol,and high density lipoprotein cholesterol(HDL-C) in does with gangrenous mastitis compared to healthy does.Moreover,there was a significant increase in the levels of malondialdehyde and uric acid with a significant decrease in the levels of reduced glutathione,super oxide dismutase,and catalase in does with gangrenous mastitis compared to healthy does.In addition,there was a significant increase in the haptoglobin,serum amyloid A,fibrinogen,interleukin 6(IL-6),IL-1β,and tumor necrosis factor-α(TNF-α) in does with gangrenous mastitis compared to healthy ones.Conclusively,oxidative stress biomarkers,acute phase proteins,and proinflammatory cytokines play an essential task as biomarkers for gangrenous mastitis in does.Mastitis may be considered as one of the ketotic stressors in does after parturition.

关 键 词：HAPTOGLOBIN Serum amyloid A MALONDIALDEHYDE Interleukin 1β Interleukin 6 Tumor necrosis factor-a 

分 类 号：R655.8[医药卫生—外科学]