慢性砷暴露对雌性大鼠心肌雌激素受体结合位点相关抗原9和雌激素反应指蛋白mRNA表达的影响  

Effects of chronic arsenic exposure on estrogen receptor-binding fragment-associated gene 9 and estrogen- responsive finger protein

作　　者：郭志伟 杨卫红[2] 韩晓红[2] 夏雅娟 

机构地区：[1]内蒙古地方病防治研究中心,呼和浩特010031 [2]内蒙古医科大学

出　　处：《中华地方病学杂志》2013年第1期24-26,共3页Chinese Journal of Endemiology

基　　金：国家自然科学基金（30960336）;内蒙古卫生厅项目（2010017）

摘　　要：【摘要】目的观察慢性砷暴露对雌性大鼠心肌雌激素受体结合位点相关抗原9（Ebag9）和雌激素反应指蛋白（efp）mRNA表达的影响。方法将50只雌性Wistar大鼠按体质量随机分成5组，每组10只，分别饮用含砷（三氧化二砷）量为0．00（对照）、O．05、0．10、0．20、0．40mg／L的水溶液，32周后通过实时定量PCR方法检测大鼠心肌中Ebag9和efpmRNA的表达。结果0．00、0．05、O．10、O．20、O．40mg／L组大鼠心肌Ebag9、efpmRNA表达量分别为0．54±0．14、0．52±0．10、0．48±0．24、0．58±0．13、0．45±0．19和0．85±0．14、0．86±0．12、0．87±0．09、0．99±0．10、0．86±0．19。与对照组比较，0．20mg／L组Ebag9mRNA表达有升高趋势，而其他各染砷组有降低趋势，但差异均无统计学意义（P均〉0．05）。与对照组比较，0．20mg／L组efpmRNA表达明显升高（P〈0．05），而其他各染砷组有升高趋势，但差异均无统计学意义（P均〉0．05）。结论慢性砷暴露可引起雌性大鼠心肌Ebag9和efDmRNA表达改变。砷可能对雌性大鼠心肌存在内分泌干扰效应。Objective To observe the effects of chronic arsenic exposure on estrogen receptor-binding fragment-associated gene 9 （Ebag9） and estrogen-responsive finger protein （efp） mRNA expression in female rat＇s myocardium. Methods Fifty female Wistar rats were randomly divided into five groups according to arsenic （As203） concentrations in drinking-water： 0.00（control）, 0.05, 0.10, 0.20, 0.40 mg/L groups and RT-PCR was used to detect Ebag9 and efp mRNA expression of myocardium at the 32 weeks of experiment. Results Ebag9 and efp mRNA expression levels in 0.00, 0.05, 0.10, 0.20, 0.40 mg/L groups were respectively as follows： 0.54 ＋ 0.14, 0.52 ±0.10, 0.48 ± 0.24, 0.58 ± 0.13, 0.45 ± 0.19 and 0.85 ± 0.14, 0.86 ± 0.12, 0.87 ± 0.09, 0.99 ± 0.10, 0.86 ±0.19. Compared to the control group, Ebag9 mRNA level of the 0.20 mg/L group was increased, and decreased in other groups, but the difference between two groups was not significant（all P 〉 0.05）. Compared to control group, the efp mRNA level of 0.20 mg/L group increased significantly（P 〈 0.05）, and showed increased tendency in other arsenic groups, but the difference between two groups was not significant （all P 〉 0.05 ）. Conclusions Ebag9 and efp mRNA expression have changed in myocardium of rats exposed to chronic arsenic. Arsenic may has endocrine disruptor effect to female rat＇s myocardium.

关 键 词：砷 心肌 雌激素受体结合位点相关抗原9 雌激素反应指蛋白 

分 类 号：R114[医药卫生—卫生毒理学]