蓝莓对肝纤维化大鼠PPARγ和PDGF—B表达的影响  

Effects of blueberry on the expression of PPARγ and PDGF-B in rat hepatic fibrosis

作　　者：王豫萍[1] 程明亮[1] 张宝方[1] 吴君[1] 

机构地区：[1]贵阳医学院附属医院感染科,550004

出　　处：《中华医学杂志》2013年第3期218-221,共4页National Medical Journal of China

基　　金：国家级教学团队经费课题（教高函[2009]12号）;贵州省科技厅中药现代化项目（黔科合ZY字[2012]3017号）;贵州省省长基金（黔省专合字（2012）44号）

摘　　要：目的观察蓝莓对肝纤维化大鼠过氧化物酶体增殖物激活受体γ（PPARγ）和血小板源性生长因子-B（PDGF-B）表达的影响。方法SD大鼠随机分为对照组、模型组、蓝莓汁预防组（BB组）、丹芍化纤预防组（DSHX组）及蓝莓汁加丹芍化纤预防组（BB＋DSHX组），每组9只。除对照组外，用CCl4复合因素复制大鼠肝纤维化模型，共8周。造模同时，预防组分别予蓝莓汁（1．5g/100g）、丹芍化纤（0．1g／100g）及蓝莓汁＋丹芍化纤（1．5g／100g＋0．1g／100g）每天灌胃1次。8周后分别采用实时荧光定量RT—PCR、免疫组织化学法及Western印迹分析大鼠肝组织I型胶原（ColⅠ）、PPARγ及PDGF-B mRNA和蛋白的表达。结果模型组肝组织PPARγ蛋白的表达明显低于对照组（P〈0．05），ColⅠ mRNA、蛋白及PDGF-B蛋白的表达均明显高于对照组（P〈0．05）；各预防组肝组织PPARγ蛋白的表达均明显高于模型组（P〈0．05），ColⅠ mRNA、蛋白及PDGF-B蛋白的表达均明显低于模型组（P〈0．05）。结论蓝莓对大鼠肝纤维化的抑制作用可能与增强PPARγ的活性，从而抑制细胞因子PDGF—B的合成，减少ECM的生成有关。Objective To explore the effects of blueberry on the expressions of peroxisome proliferator-activated receptor γ （PPARγ） and platelet-derived growth factor B （PDGF-B） in rat hepatic fibrosis. Methods A total of 45 male Sprague-Dawley rats were randomly divided into control group, CCl4- induced hepatic fibrosis （ model group）, blueberry prevention （ BB group）, DSHX prevention （ DSHX group） and blueberry ＋ DSHX prevention （ BB ＋ DSHX group） （ n = 9 each）. Fibrous liver models of rats were induced by subcutaneous injection of CCl4 and high-lipid/low-protein diet for 8 weeks except for control group. Then the expressions of collagen Ⅰ （ Col Ⅰ ）, PPARγ, and PDGF-B were determined by real-time polymerase chain reaction （RT-PCR）, immunohistochemistry and Western blot respectively. Results Compared with the control group, the expression of PPARγ decreased and those of Col Ⅰ and PDGF-B were elevated in model group （P 〈 0. 05 ）. The expression of PPARγ, increased and Col Ⅰ and PDGF-B decreased in BB, DSHX and BB ＋ DSHX groups as compared to model group （P 〈 0.05）. Conclusion The inhibitory effects of blueberry in CCl4-induced liver fibrosis may be correlated with the activation of PPARγ, the inhibited expression of PDGF-B and the reduced synthesis of extracellular matrix.

关 键 词：肝硬化 过氧化物酶体增殖物激活受体 血小板源性生长因子 胶原Ⅰ型 

分 类 号：R285.5[医药卫生—中药学]