曲尼司特对糖尿病肾病大鼠肾脏的保护作用  

Protective Effects of Tranilast on Diabetic Nephropathy in Rats

作　　者：王晓慧 张新 何泳 杨国胜 路静芬 李静 张斌 

机构地区：[1]武汉市第五医院肾内科,湖北武汉430050

出　　处：《武汉大学学报（医学版）》2013年第1期42-45,共4页Medical Journal of Wuhan University

基　　金：武汉市卫生局科研项目(编号:WX10C24)

摘　　要：目的:研究曲尼司特对糖尿病肾病大鼠肾脏保护作用,并探讨其可能的机制。方法:雄性SD大鼠30只,随机分为正常对照组、糖尿病肾病模型组、曲尼司特治疗组。观察各组大鼠24 h尿蛋白定量、血糖、血尿素氮、血肌酐及肾脏病理的变化。应用RT-PCR方法和Western-blot方法测定肾组织硫氧还蛋白(Trx)mRNA和蛋白水平的表达。结果:曲尼司特能减少糖尿病肾病大鼠的尿蛋白,减轻肾小球纤维化,糖尿病模型组肾组织中Trx表达下降,曲尼司特治疗组大鼠肾组织中Trx的表达上升。结论:曲尼司特可能通过上调糖尿病肾病大鼠组织中Trx的表达,调节细胞外基质的形成,从而减轻肾脏病理损害,起到保护肾脏的作用。Objective： To investigate the protective effects of tranilast on diabetic nephropathy in rats, and to explore its possible mechanism. Methods： Thirty male SD rats were randomly divided into normal control group, model group, and tranilast treated group. The changes of 24-hour urinary protein excretion, blood glucose, nitrogen （BUN）, serum ereatinine （Scr）, and renal pathology were observed in each group. Thioredoxin （Trx） mRNA and protein expression on renal tissue were studied with RT-PCR and Western blot. Results. Tranilast could reduce the urinary protein of rats with diabetic nephropathy and delay glomerular fibrosis. The expression level of Trx was reduced in diabetic nephropathy group while increased in tranilast treated group. Conclusion： Tranilast might up-regulate Trx expression on the tissue of diabetic nephropathy, regulate extracellular matrix formation, so as to reduce the renal pathological damage and play the role of protecting the kidney.

关 键 词：曲尼司特 糖尿病肾病 硫氧还蛋白 

分 类 号：R587.1[医药卫生—内分泌]