改性胶原支架构建组织工程化神经复合物修复大鼠脊髓损伤  

Tissue-engineered neural complex seeded in modified collagen scaffold for treatment of acutespinal cord injury in adult rats

作　　者：蒲渝[1] 郭庆山[2] 王爱民[3] 王子明[3] 

机构地区：[1]解放军第三二四医院骨科,重庆400020 [2]第三军医大学附属大坪医院野战外科研究所全军战创伤中心创伤外科 [3]第三军医大学附属大坪医院野战外科研究所全军战创伤中心骨科

出　　处：《中华创伤杂志》2013年第1期67-72,共6页Chinese Journal of Trauma

摘　　要：目的利用组织工程技术体外构建组织工程化神经复合物，对大鼠损伤脊髓进行修复并观察其效果。方法（1）分离培养大鼠神经干细胞（neuralstemcells，NSCs），制备改性胶原支架，体外构建NSCs／改性胶原支架复合物，以胚胎脊髓提取液对NSCs进行诱导分化，通过组织化学检测和扫描电镜观察其结构。（2）制作大鼠脊髓半横断损伤模型，植入组织工程化神经复合物，术后每2周进行功能学检查，并于术后3周及8周进行组织学检查以评估修复脊髓损伤（spinalcordinjury，SCI的效果。结果改性胶原支架构建的组织工程化神经复合物移植至大鼠体内后，其种子细胞存活并进一步分化，形成功能活跃的神经元，分化的胶质细胞形成规则的网状结构。大鼠损伤脊髓平面以下运动功能获得了较明显的恢复。结论以改性胶原为支架的组织工程化神经复合物对大鼠损伤脊髓的结构重建和功能恢复都有一定的作用。Objective To repair the injured spinal cord in adult rats using tissue-engineered neural complex constructed in vitro by tissue engineering techniques and discuss the treatment effect. Methods Neural stem cells （NSCs） from rats were cultured separately and modified collagen scaffold was also prepared to construct complex of NSCs /modified collagen scaffold in vitro. Embryo spine cord extract was administered to induce differentiation of NSCs, the structure of which was revealed by histochemical technique and scanning electron microscope. The rat models of hemisected spinal cord injury were built and grafted with tissue-engineered neural complex. Functional recovery of the models was evaluated every two weeks postoperatively. Effect in repair of spinal cord injury was assessed by histological method at postoperative three and eight weeks. Results After tissue-engineered neural complex planted in the modified collagen scaffold was grafted into the injured spinal cord in adult rats, the seed cells survived and further differentiated into functionally active neurons. The differentiated glia cells presented quite regular reticular structure. Motor function below the level of the injured spinal cord in adult rats obtained obvious recovery. Conduslon Tissue-engineered neural complex seeded in the modified collagen scaffold takes effect in structural reconstruction and functional recovery of the injured spinal cord in adult rats.

关 键 词：脊髓损伤 组织工程 神经干细胞 胶原 

分 类 号：R651.2[医药卫生—外科学]