Morris水迷宫测评方法的改进及对快速老化痴呆小鼠SAMP8认知功能的再评价  

Improvements of Morris Water Maze Evaluation Method and Re-evaluation of the Rapid Aaging Dementia SAMP8 Cognitive Function in Mice

作　　者：刘金凤[1] 聂坤[2] 栗振杰[1] 徐思思[1] 张莹[1] 孙金平[1] 张雪竹[2] 

机构地区：[1]天津中医药大学研究生院,天津300193 [2]天津中医药大学第一附属医院,天津300193

出　　处：《中国中医急症》2013年第1期5-7,22,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然基金课题(81202739;81072882);教育部博士点课题(20111210120004);天津市教委课题(20080217)

摘　　要：目的改进Morris水迷宫的测评方法，使之更适于评价小鼠的认知功能．并对快速老化痴呆小鼠SAMP8的认知功能进行再评价。方法以SAMP8组及其正常同源对照小鼠SAMR1为研究对象．优化Morris水迷宫的测评条件，研究人水点位置、每日游泳次数、试验次序、颜色等对其空间参考记忆能力的影响。结果SAMP8小鼠在各部分试验的逃避潜伏期明显长于SAMR1小鼠；SAMP8小鼠比SAMRl小鼠的游泳速度慢，自身游泳速度在试验过程中基本保持稳定；在改变试验方法的第1日，两组潜伏期没差异；SAMP8小鼠的搜索策略多为随机式和边缘式，SAMRl小鼠多为直线式和趋向式。结论将探索试验穿插于隐蔽和反向平台试验中更能有效的检验动物的短时学习记忆能力；不能把改变试验方法后第1日的成绩作为评价小鼠学习记忆能力的标准；每日4～5次的训练次数不会影响小鼠的游泳速度；SAMP8小鼠是研究年龄相关学习和记忆缺陷较好的动物模型。Objective： To improve evaluation of the Morris water maze,which make it more suitable for the evaluation of the cognitive function of mice. And re-evaluate the cognitive function of the rapid aging dementia mice the SAMP8 （senescence accelerated mouse prone 8）. Methods： The SAMP8 group and its homologous normal control mice SAMR1 （senescence accelerated mouse resistant 1 ） were taken as the research object,and the evaluation of the Morris water maze conditions was optimized. The effect of the mice spatial reference memory capacity by into water point position,the number of daily swim,test order,color and ect were researched. Results： The SAMP8 mice escape latency in each part of the test significantly was longer than SAMR1 mice. SAMP8 mice swam slower than SAMR1 mice. SAMP8 mice their own swimming speed remained stable during the test. Groups latency show no difference on the first day of change the test method.The SAMP8 mice search strategy were mostly random type and edge-type while the SAMR1 mice were linear type and tend type. Conclusion： Explore trials interspersed in hidden and reverse platform test is more effective in test mice short-term learning memory abilities. The scores of the first day after changing test method can not be the standard of evaluate the learning and memory abilities of mice. 4-5 times a day,the number of training does not affect the swimming speed of the mice. SAMP8 mice is a good animal model to study age-related learning and memory deficits.

关 键 词：Morris水迷 宫阿尔兹海默症 方法 记忆 

分 类 号：R285.5[医药卫生—中药学]