HPLC法测定斑秃搽剂中米诺地尔含量  

Determination of minoxidil content in liniment for alopecia areata by HPLC method

作　　者：赵瑛[1] 谢军[1] 廖海燕[2] 

机构地区：[1]成都军区总医院药剂科,成都610083 [2]成都军区药品仪器检验所

出　　处：《西南国防医药》2013年第1期29-31,共3页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的应用HPLC法建立斑秃搽剂中米诺地尔含量测定方法。方法对某医院提供的3批次斑秃搽剂,检测其中米诺地尔的含量。采用SHMADZU VP-ODS色谱柱,流动相:甲醇-水(72∶28),检测波长230 nm,柱温35℃。结果米诺地尔对照品线性方程式为Y=153.37X-42.331(n=6),r=0.9999;样品平均回收率为101.94%,RSD=0.6%(n=6);精密度良好,RSD=0.9%(n=5)。结论 HPLC法能准确测定出斑秃搽剂中米诺地尔含量,可作为该制剂的质量控制检测方法。Objective To establish the method for the detection of minoxidil content in alopecia areata liniment by HPLC. Methods The content of minoxidil in three batches of alopecia areata liniment supplied by a hospital were detected by SHMADZU VP - ODS chromatographic column with the mobile phase of methanol - water（72：28 ）, the detecting wavelength of 230 nm, and the column temperature of 35 ℃. Results The linear equation of minoxidil reference substance was Y = 153.37X - 42.331 （ n = 6）, r = 0.9999. The sample average recovery rate was 101.94% and RSD was 0.6% （ n = 6）. The precision was good, and RSD was 0.9% （n = 5 ）. Conclusion The method of HPLC can accurately detect the content of minoxidil in alopecia areata liniment, which can be used as the method for the quality control of this liniment.

关 键 词：斑秃搽剂 米诺地尔 含量 HPLC 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]