消毒方法对钛种植体表面TiO_2纳米管的蛋白质吸附能力的影响  

The effects of sterilization method on the protein adsorption capacity of TiO_2 nanotubes on the surface of titanium implants

作　　者：孙彦维[1] 吕武龙[1] 张振庭[2] 王娜[2] 李彦秋[1] 

机构地区：[1]天津医科大学口腔医院,天津300070 [2]首都医科大学附属北京口腔医院,北京100050

出　　处：《北京化工大学学报（自然科学版）》2013年第1期93-97,共5页Journal of Beijing University of Chemical Technology(Natural Science Edition)

摘　　要：研究了紫外线照射、高压蒸汽灭菌处理对钛表面TiO2纳米管亲水性及蛋白质吸附的影响。纯钛试件经过打磨形成光滑钛,光滑钛在20 V电压下进行阳极氧化形成80～100 nm的TiO2纳米管。对TiO2纳米管及光滑钛进行紫外线照射、高压蒸汽灭菌处理后测量表面接触角,然后在37℃下进行牛血清白蛋白(BSA)的吸附及释放。结果表明:紫外线照射组的TiO2纳米管接触角较小,亲水性较好;紫外组蛋白质吸附多于高压组,而高压组的蛋白质释放快于紫外组。两种消毒方法综合比较,TiO2纳米管紫外线照射优于高压蒸汽灭菌。The aim of this study was to compare the effects of uhraviolent irradiation and autoclaving on the wetta- bility and protein adsorption of TiO2 nanotubes on the surface of titanium. Pure titanium sheets were polished with SiC abrasive paper. The polished titanium sheets were anodized under a voltage of 20 V, which resulted in a nano- tubular topography with a tube diameter of 80 - 100 nm on the surface of titanium （NT-Ti）. The samples were then sterilized with UV irradiation or autoclaving. The results of contact angle measurements indicated that the con- tact angles of NT-Ti sterilized with UV irradiation were smaller than those of NT-Ti sterilized with autoclaving, showing that NT-Ti sterilized by UV irradiation was more hydrophilic than that sterilized by autoclaving. The ad- sorption behavior of bovine serum albumin （BSA） on the nanotube arrays was investigated at 37 ℃. The release ex- periment results suggested that protein adsorption on the UV irradiated materials was greater than that for the auto- claved materials, while protein release from the autoclaved materials was faster than that from the UV irradiated ma- terials. Therefore it is recommended that such implants are sterilized by UV irradiation in the future.

关 键 词：消毒 TIO2纳米管 亲水性 牛血清白蛋白 蛋白质吸附及释放 

分 类 号：R318.08[医药卫生—生物医学工程]