龙凤竹愈伤组织诱导及再分化的试验  

Experiment on callus induction and redifferentiation of Pedilanthus tithymaloides var.nanus

作　　者：袁云香[1] 

机构地区：[1]渭南师范学院化学与生命科学学院,陕西渭南714000

出　　处：《林业科技开发》2013年第1期108-110,共3页China Forestry Science and Technology

基　　金：陕西省教育厅项目(编号:12JK0832);陕西省教育厅项目(编号:09JK434);国家自然科学基金项目(编号:31000410)

摘　　要：以龙凤竹茎段为外植体,采用不同培养基进行愈伤组织诱导试验。将获得的龙凤竹愈伤组织转入再分化培养基中,采用正交设计实验,添加不同浓度6-BA、NAA和蔗糖,组成9种不同的再生植株分化培养基,对龙凤竹愈伤组织进行再分化培养。结果表明:NMB为最适的基本培养基;诱导培养基中添加6-BA 1 mg/L+NAA0.1 mg/L时,有利于龙凤竹愈伤组织形成,诱导率达86.67%;再分化率最高的培养基配方为NMB+6-BA2.0 mg/L+NAA 0.2 mg/L+5%蔗糖。The stems of Pedilanthus tithymaloides var.nanus were taken as explant and induced callus formation.The different culture media,the proportion of different plant hormone were investigated.To add different concentration 6-BA and NAA to the medium composed of 9 different plant regeneration medium by orthogonal design L9（34）were investigated.The results showed that the suitable media NMB was in favor of the induction,the induction medium added 1 mg/L 6-BA and 0.1 mg/L NAA was beneficial to the callus induction.The suitable regeneration medium was NMB containing 2.0 mg/L 6-BA,0.2 mg/L NAA and 5% sucrose,it could improve obviously the frequency of regenerated shoots.

关 键 词：龙凤竹 组织培养 愈伤组织 再分化 

分 类 号：S567.239[农业科学—中草药栽培]