喘可治抑制哮喘大鼠炎症反应的机制研究  

Mechanisms of “Chuankezhi Injection” in suppressing inflammatory reaction in asthmatic rats

作　　者：夏敏[1] 蔡宇波[1] 费辛[1] 曹兰芳[1] 

机构地区：[1]上海交通大学医学院附属仁济医院儿科,上海200001

出　　处：《上海中医药杂志》2013年第1期70-73,共4页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市科委资助项目(08411953100)

摘　　要：目的探讨喘可治抑制哮喘大鼠炎症反应的可能机制。方法选用Wistar大鼠90只,其中15只为正常对照组,另75只制作哮喘大鼠模型并随机分为用不同剂量喘可治治疗组、地塞米松治疗组、模型组,共5组。各组给予相应的处理措施,采用酶联免疫吸附法测定各组大鼠外周血脂氧素A4(LXA4)、巨噬细胞移动抑制因子(MIF)、白介素-4(IL-4)、γ-干扰素(IFN-γ)含量,采用流式细胞仪测定淋巴细胞亚群比例。结果与正常组比较,模型组除CD4+CD25+T调节细胞外,其余指标差异均有统计学意义(P<0.05)。高剂量喘可治治疗组大鼠血清LXA4、INF-γ浓度均明显增高,且高于中、低剂量喘可治和地塞米松治疗组,差异有统计学意义(P<0.05)。各剂量喘可治治疗组MIF浓度均较模型组明显下降,差异有统计学意义(P<0.05)。各剂量喘可治治疗组IL-4浓度均较模型组明显下降,其中高剂量组下降最明显,差异有统计学意义(P<0.05)。高、中、低剂量喘可治治疗组CD4+CD25+T调节细胞百分比均明显上升,其中高剂量组升高最明显,与模型组比较差异有统计学意义(P<0.05)。高、中剂量喘可治治疗组CD3-CD161a+NK细胞的百分比明显上升,与模型组、低剂量组比较差异有统计学意义(P<0.05)。结论高剂量喘可治腹腔注射可能影响哮喘模型大鼠外周血LXA4、MIF、IL-4、IFN-γ等多种抑炎因子或细胞因子的浓度,缓解哮喘大鼠的炎症反应,同时可能影响哮喘模型大鼠外周血Treg细胞和NK淋巴细胞亚群的比例,有利于预防和控制哮喘。Objective To investigate the possible mechanisms of ＂Chuankezhi Injection＂ in the treatment of asthma. Methods Fifteen of 90 rats were used as normal group and the other 75 rats were modeled to be asthmatic, and then randomized into five group ： different dosages of ＂Chuankezhi Injection＂ groups, dexam- ethasone group and model group. The contents of LXA4, MIF, IL-γ and IFN-γ in the blood from the asthmatic rats were measured by ELISA, and the proportions of CIM ＋ CD25 ^＋T cell and CD3^＋CD161a ^＋ NK T cell were detected by FCM. Results Except for CD4^＋ CD25 ^＋T, there were some differences in the other indexes between the normal and model group（P 〈 0.05 ）. In the high-dose ＂Chuankezhi Injection＂ group, the serum LXA4 and IFN-γ concentration were significantly increased, higher than those in the middle- and low-dose ＂Chuankezhi Injection＂ group and dexamethasone group（ P 〈 0.05 ）. In ＂Chuankezhi Injection＂ groups, the MIF concentrations were significantly lowered in comparison with the model group （ P 〈 0.05 ） ; the IL-4 concentration was significantly decreased in comparison with the model group, with most significant decrease in the high-dose ＂ Chuankezhi Injection＂ group （ P 〈 0.05 ）. In the ＂ Chuankezhi Injection＂ groups, the CD4 ^＋ CD25 ^＋ T cell percentages were significantly increased, especially in the high-dose ＂Chuankezhi Injection＂ group and with higher percentage than the model group（P 〈 0.05）. In high- and middle-dose ＂Chuankezhi Injection＂ groups, the CDTCD161 a ^＋ NK T cell percentages were increased obviously, higher than those in the model group and low-dose ＂Chuankezhi Injection＂ group. Conclusion ＂Chuankezhi Injection＂ can suppress inflammatol7 reactions in asthma rats by suppressing LXA4, MIF, IL-4 and IFN-γ and regulating Treg and NK cells.

关 键 词：哮喘 喘可治注射液 脂氧素A4 巨噬细胞移动抑制因子 白介素4 γ-干扰素 

分 类 号：R285.5[医药卫生—中药学]