基于UPLC特征指纹图谱和指标成分定量测定研究炮姜的炮制工艺  

Processing technology of Zingiberis Rhizoma Praeparata based on UPLC fingerprints and quantitative determination of index components

作　　者：韩燕全[1] 洪燕[2] 高家荣[1] 桂洁[2] 汪永忠[1] 夏伦祝[1] 郑礼胜[3] 

机构地区：[1]安徽中医学院第一附属医院国家中医药管理局中药制剂三级实验室,安徽合肥230031 [2]安徽中医学院,安徽合肥230031 [3]天津药物研究院,天津300193

出　　处：《中草药》2013年第1期42-46,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81102811);国家中医药重点学科临床中药学建设项目(国中医药人教发[2012]32号);安徽省自然科学基金资助项目(10040606Q40)

摘　　要：目的从UPLC特征指纹图谱和6-姜酚、8-姜酚、10-姜酚量的角度,选择炮姜的最佳炮制温度和炮制时间。方法采用砂烫法,选取180、185、190、195、200℃,每个温度下分别炒制6、7、8 min,共得到15份样品;采用超高效液相色谱(UPLC)法分析各样品指纹图谱相似度;同时采用UPLC法测定不同炮制品中6-姜酚、8-姜酚、10-姜酚的量。结果 195℃、7 min和195℃、8 min 2份样品的相似度最大,分别为0.995和0.994;6-姜酚、8-姜酚、10-姜酚的总量最低,分别为5.792、5.412 mg/g。结论综合指纹图谱相似度和6-姜酚、8-姜酚、10-姜酚量的测定结果以及炮制品外观,确定炮姜砂烫炮制以195℃、7 min为宜。Objective To optimize the processing temperature and time of Zingiberis Rhizoma Praeparata （ZRP） according to ultra- high performance liquid chromatography （UPLC） fingerprints and the contents of 6-gingerol, 8-gingerol, and 10-gingerol. Methods Samples were treated by stir-baking in sand method at 180, 185, 190, 195, and 200 ℃, stirred for 6, 7, and 8 min at each temperature, respectively. Then the fingerprint similarity of the samples and the contents of 6-gingerol, 8-gingerol, and 10-gingerol were determined by UPLC method. Results The similarities of the two samples at 195 ℃ for 7 min and for 8 min were the highest, 0.995 and 0.994, while the contents of 6-gingerol, 8-gingerol, and 10-gingerol of the two samples were the lowest, 5.792 and 5.412 mg/g, respectively. Conclusion According to the similarities of the fingerprint, the contents of 6-gingerol, 8-gingerol, 10-gingerol, and the apperance of the processed products, ZRP should be better processed by stir-baking in sand method at 195 ℃ for 7 rain.

关 键 词：UPLC 指纹图谱 炮姜 姜酚 砂烫法 

分 类 号：R286.02[医药卫生—中药学]