黄芩苷对小鼠巨噬细胞CD36表达及肿瘤坏死因子-α分泌的影响  

Effects of Baicalin on CD36 Expression and Secretion of Tumor Necrosis Factor Alpha in Mice Macrophages Stimulated by Lipopolysaccharide

作　　者：李岩[1] 孙冬梅[1] 万幸[1] 马嫚[1] 李明[2] 陈现红[3] 邝枣园[1] 

机构地区：[1]广州中医药大学基础医学院微生物学与寄生虫学教研室,广东广州510006 [2]广东药学院基础学院病理生理学教研室,广东广州510006 [3]广州军区广州总医院康复科,广东广州510010

出　　处：《广州中医药大学学报》2013年第1期55-58,129,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:30901980);广东省自然科学基金资助项目(编号:10151022401000000);广东省自然科学基金博士启动基金项目(编号:9451001002003887)

摘　　要：【目的】观察黄芩苷对脂多糖(lipopolysaccharide,LPS)诱导的小鼠巨噬细胞CD36表达及炎症因子肿瘤坏死因子-α(TNF-α)分泌的影响。【方法】分别用LPS(终浓度0.1μg/mL)或LPS加黄芩苷(终浓度分别为50、100μmol/L)处理生长良好的小鼠巨噬细胞RAW264.7,采用实时荧光定量PCR法和流式细胞术检测黄芩苷对巨噬细胞CD36分子表达的影响,然后采用酶联免疫吸附(ELISA)法检测细胞上清液中TNF-α含量变化。【结果】LPS刺激可以诱导巨噬细胞CD36表达升高,与空白对照组比较差异有统计学意义(P<0.01);不同浓度黄芩苷预处理组可显著抑制LPS诱导的CD36基因转录和蛋白表达,与LPS组比较差异有统计学意义(P<0.01);黄芩苷还可显著减少巨噬细胞炎症因子TNF-α的分泌(P<0.05)。【结论】黄芩苷可通过抑制CD36表达,下调LPS诱导的巨噬细胞炎症因子TNF-α的生成从而发挥抗炎作用。Objective To investigate the influence of baicalin on the expression of CD36 and the secretion of tumor necrosis factor alpha（TNF-α） in mice macrophages induced by lipopolysaccharide（LPS）.Methods RAW264.7 cells were cultured in vitro and treated with different concentrations of baicalin（with final concentration being 50,100 μmol/L） in the presence or absence of LPS（0.1 μg/mL）.The expression of CD36 mRNA and protein was determined by real-time fluorescence quantitative polymerase chain reaction（PCR） and flow cytometry assay,respectively.The change of inflammatory factor TNF-α in the supernatant of RAW264.7 cells was detected by enzyme-linked immunosorbent assay（ELISA）.Results The expression of CD36 was significantly up-regulated in RAW264.7 cells stimulated by LPS（P〈0.01 compared with that in the blank control group）.The levels of CD36 mRNA and protein in LPS-induced RAW264.7 cells were decreased by pretreatment of baicalin（P〈0.01 compared with those in LPS group）.Meanwhile,the secretion of TNF-α was reduced under the same condition（P0.05）.Conclusion Baicalin can effectively inhibit the expression of CD36 and reduce the secretion of TNF-α in LPS-induced RAW264.7 cells,and through which to exert anti-inflammatory action.

关 键 词：黄芩苷 药理学 动脉粥样硬化 中药疗法 细胞因子 基因表达调控 细胞培养 

分 类 号：R285.5[医药卫生—中药学]