利用植物细胞组织体外生产外源蛋白的生物反应器系统  

Bioreactor Systems for Production of Foreign Proteins Using Plant Tissue in Vitro

作　　者：李玉洁[1] 

机构地区：[1]上海交通大学生命科学技术学院植物发育生物学研究室,上海200240

出　　处：《广东化工》2013年第2期46-47,10,共3页Guangdong Chemical Industry

摘　　要：用在过去的20年间,植物细胞被认为是外源蛋白表达的高效宿主(利用整株植物或是体外植物细胞组织)。最近几年,植物细胞悬浮培养的生物反应器已开始代替传统的微生物发酵和哺乳动物细胞培养。因植物细胞生物反应器具有无限生产重组蛋白的巨大潜力。利用转基因植物表达的重组蛋白具备原有的理化性质和生物活性,从而为人类提供了一种大量生产药用蛋白的安全可靠、经济、方便的新生产体系。研究证明,用各种农作物为载体的植物生物反应器产品可通过种子、果实或块茎表达,便于贮藏、运输和利用。目前已广泛运用于工业、农业尤其是生命科学以及医学制造领域。因此它具有广阔的市场前景和商业价值。综述植物生物反应器的研究与应用现状,并对植物生物反应器的发展前景作分析和展望。Plant cells have been demonstrated to be an attractive heterologous expression host （using whole plants and in vitro plant cell cultures） for foreign protein production in the past 20 years. In recent years in vitro liquid cultures of plant cells in a fully contained bioreactor have become promising alternatives to traditional microbial fermentation and mammalian cell cultures as a foreign protein expression platform, due to the unique features of plant cells as a production host including product safety, cost-effective biomanufacturing, and the capacity for complex protein post-translational modifications. Heterologous proteins such as therapeutics, antibodies, vaccines and enzymes for pharmaceutical and industrial applications have been successfully expressed in plant cell culture-based bioreactor systems including suspended dedifferentiated plant cells, moss, and hairy roots, etc. In this article, the current status and emerging trends of plant cell culture for in vitro production of foreign proteins will be discussed with emphasis on the technological progress that has been made in plant cell culture bioreactor systems.

关 键 词：外源蛋白 植物细胞组织 生物反应器 

分 类 号：TQ[化学工程]