一种改进的苹果总RNA高效提取方法  

An Improved Efficient Method for Total RNA Extraction from Apple

作　　者：杨凤秋[1] 高燕[2] 曾广娟[3] 李春敏[1] 陈东玫[1] 赵永波[1] 张新忠[2] 

机构地区：[1]河北省农林科学院昌黎果树研究所,河北昌黎066600 [2]中国农业大学农学与生物技术学院,北京100094 [3]中国环境管理干部学院,河北秦皇岛066004

出　　处：《河北农业科学》2012年第6期24-26,共3页Journal of Hebei Agricultural Sciences

摘　　要：在改进的CTAB提取法基础上,结合总RNA的LiCl沉淀法,摸索出了一套适合苹果组织总RNA提取和纯化的方法。该方法得到的苹果总RNA完整性好,纯度和得率高,且成本低,容易操作。解决了RNA易降解以及木本植物组织中多酚和多糖含量高导致的在提取过程中RNA被氧化和共沉淀从而导致RNA产率低的问题。Based on the improvement of CTAB extraction method, and combining the LiCL precipitation methed of total RNA, a set of method suitable for apple tatol RNA extraction and purification was built, which was simple and low-cost, and the total RNA received with high-quality and high-quantity. The problems of RNA degradation and low yield of RNA attributed to oxidation by polyphenol and coprecipitation with polysaccharides were solved.

关 键 词：苹果 总RNA 分离 提取方法 

分 类 号：Q943[生物学—植物学]