不同标本来源大肠埃希菌分布菌毛及基因分型研究  

Study on distribution and genotyping of fimbriae and pili of Escherichia coli from various specimens

作　　者：陈圣高 刘洋[2] 李向阳[3] 

机构地区：[1]浙江省平阳县中医院检验科,浙江平阳县325401 [2]南昌大学第一附属医院检验科,江西南昌330006 [3]温州医学院附属第二医院检验科,浙江温州325400

出　　处：《中华医院感染学杂志》2013年第2期245-247,共3页Chinese Journal of Nosocomiology

基　　金：温州市医药卫生科学研究项目(2011B091)

摘　　要：目的调查医院不同标本分离大肠埃希菌P菌毛基因分型,探究基因分型与标本来源之间的关系。方法收集2009年1-12月温州医学院附属第二医院分离大肠埃希菌120株,采用Microscan Walk away 96SI微生物鉴定仪进行鉴定和药敏试验,按CLSI 2009年标准判定;用PCR法检测P菌毛和I型菌毛基因;采用χ2检验,P<0.05为差异有统计学意义。结果 papC基因阳性率为39.19%,其中尿液分离株阳性率为54.50%,显著高于痰液、引流液以及脓液分离株(P<0.05);papG分型率为64.20%,papGⅡ型检出率为46.67%,其中尿液分离株阳性率为70.54%,显著高于痰液和血液分离株(P<0.05);papGⅢ型检出率为17.50%,其中尿液分离株阳性率为25.00%,显著高于痰液分离株(P<0.05);未检测出papGⅠ型。结论不同标本分离大肠埃希菌papC基因携带率不同,尿液中肾盂肾炎大肠埃希菌(UPEC)的分离率最高,papG分型以papGⅡ型为主,存在部分papGⅢ型。OBJECTIVE To investigate the P pilus genotyping of Escherichia colt isolated from various specimens, and explore the relationship between genotyping of P pilus and specimens. METHODS A total of 120 strains of E. colt isolated from Second Affiliated Hospital of Wenzhou Medical College from Jan to Dec 2009 were collected, P pill and type I pilus gene were determined by PCR. RESULTS The positive rate of papC gene was 39.19%, and the positive rate of the isolates from urine was 54. 5%, which was significantly higher than that of the isolates from sputum, drainage, and pus（P〈0. 05）. The genotyping of PapG showed only 64. 2% strains could be divided into three papG genotypes, the rate of papG Ⅱ genotype was 46.67%, in the isolates from urine, the positive rate of papGⅡ genotype was 70.54%, which was significantly higher than that of the isolates from the sputum and blood （P〈0.05）; the rate of papG Ⅲ genotype was 17.50%, and the rate of papG Ⅲ genotype in urine isolates was 25.00%, which was significantly higher than that of the sputum isolates （P〈0.05） ; no papG I genotype was detected. CONCLUSION The positive rate of papC gene is different among different specimens in E. colt, the isolation rate of uPEc from urine isthe highest. The papGⅡ genotype is the main type of papG, with papGⅢ genotype existing in some of the strains.

关 键 词：大肠埃希菌 肾盂肾炎大肠埃希菌 菌毛 基因分型 

分 类 号：R378.21[医药卫生—病原生物学]