两种血培养系统对细菌与真菌检测能力比较  

Two blood culture systems in detection of bacteria and fungi:a comparative study

作　　者：栗方[1] 曹彬[1] 刘颖梅[1] 黎斌斌[1] 王珊珊[1] 杨春霞[1] 王春雷[1] 

机构地区：[1]首都医科大学附属北京朝阳医院感染和临床微生物科,北京100020

出　　处：《中华医院感染学杂志》2013年第2期473-475,共3页Chinese Journal of Nosocomiology

摘　　要：目的对法国生物梅里埃公司BacT/ALERT 3D与美国Trek公司Versa TREK两种血培养系统进行性能比较。方法两种血培养系统分成需氧瓶组、厌氧瓶组和抗菌药物瓶组,将无菌血、菌液、抗菌药物按要求分别注入血培养瓶内,放入自动化仪器内进行培养,然后记录5d内阳性瓶的检出时间,试验结束后对不同系统血培养瓶检出时间进行比较。结果不含抗菌药物组大肠埃希菌(7.77±0.42/9.44±0.27)h、金黄色葡萄球菌(9.73±0.12/11.92±0.14)h、铜绿假单胞菌(9.44±0.69/12.48±0.00)h、流感嗜血菌(10.77±0.15/15.04±0.73)h、难辨梭菌(18.00±0.87/20.88±4.34)h,Versa TREK检出时间早于BacT/ALERT 3D,差异有统计学意义(P<0.05);肺炎链球菌(17.83±0.31/15.84±2.70)h、普通拟杆菌(54.80±2.10/24.16±0.60)h,BacT/ALERT 3D检出时间早于Versa TREK,差异有统计学意义(P<0.05);不含抗菌药物组脆弱拟杆菌(21.90±0.30/21.84±1.73)h、产气荚膜梭菌(11.10±0.46/11.04±0.00)h,两种血培养系统检出时间差异无统计学意义;对含抗菌药物环丙沙星的大肠埃希菌,BacT/ALERT 3D检出时间小于Versa TREK,铜绿假单胞菌和金黄色葡萄球菌Versa TREK小于BacT/ALERT 3D;含抗菌药物头孢他啶和头孢噻肟的大肠埃希菌、铜绿假单胞菌、金黄色葡萄球菌,含抗菌药物万古霉素的金黄色葡萄球菌、屎肠球菌、肺炎链球菌、粪肠球菌,Versa TREK检出时间早于BacT/ALERT 3D,差异有统计学意义(P<0.05)。结论两种血培养系统均能有效检测由细菌和真菌引起的血流感染,Versa TREK检出病原菌时间早于BacT/ALERT 3D。OBJECTIVE To compare the performance of blood culture systems such as BacT/ALERT 3D of French bio-Merieux and Versa TREK of US in detecting bacteria and fungi. METHODS Blood culture systems were divided into the aerobic group, the anaerobic group and the antibiotics group. Bacteria, antibiotics and sterile blood blend were added to blood culture bottles. All the bottles were cultured in automations for five days, and then the time of the detection by the different blood culture bottles was compared. RESULTS Versa TREK bottles were superior in detecting bottles containing Escherichia coli （7. 77 ±0. 42/9. 44 ± 0. 27） h, Staphylococcus aureus （9.73±0.12/11. 92±0.14）h, Pseudomonas aeruginosa （9.44±0.69/12.48±0.00）h, Haemophilus influenzae （10. 77±0.15/15.04±0.73）h, Clostridium difficile （18.00±0.87/20.88±4.34）h, the detection time of the Versa TRE was earlier than that of BacT/ALERT 3D, the difference was statistically significant （P〈0.05）. BacT/ALERT bottles were superior in detecting bottles containing Streptococcus penumoniae （17. 83±0. 31/ 15.84±2.70）h and ordinary bacteroidaceae （54.80±2.10/24.16±0.60）h, the detection time of BacT/ALERT was earlier than that of Versa TREK, the difference was statistically significant （P〈0.05）; BacT/ALERT 3D needed less time than did the Versa TREK in detecting E. coli, P. aesuginosa and S. aureus in the bottles containing ciprofloxacin; there was no statistical difference in the detection time between the two blood culture systems for Bacteroides fragilis （21. 90±0.30/21.84±1.73）h and Clostridium perfringens （11.10±0.46/11.04±0.00）h. Versa TREK needed less time in detecting E. coli, S. aureus, P. aeruginosa, Enterococcus faecium, S. pneurnoniae and Enterococcus faecalis in the bottles containing ceftazidime, cefotaxime and vancomycin （P〈 0.05）. CONCLUSION Both of the two blood culture systems can effectively detect the blood stream infections caused by the bacteria or fungi, and Versa TREK needs less time t

关 键 词：血培养 检出时间 抗菌药物 

分 类 号：R446.1[医药卫生—诊断学]