尿微量白蛋白、尿β_2-微球蛋白、转铁蛋白、糖化血红蛋白联合检测在糖尿病动物模型中的应用  

Application value of combined detection of urine microalbumin,β_2-microglobulin,transferrin,glycated hemoglobin and serum lipids in macaque monkey models of diabetes mellitus

作　　者：朱华[1] 刘颖[1] 黄澜[1] 徐艳峰[1] 马春梅[1] 赵春华[2] 秦川[1] 

机构地区：[1]卫生部人类疾病比较医学重点实验室国家中医药管理局人类疾病动物模型三级实验室中国医学科学院医学实验动物研究所北京协和医学院比较医学中心,北京100021 [2]中国医学科学院基础医学研究所,北京100005

出　　处：《中国实验动物学报》2012年第6期26-30,I0004,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：863课题(2009ZX09503-025)

摘　　要：目的探讨尿微量白蛋白(mALb)、尿β2-微球蛋白(β2-MG)、尿转铁蛋白(TRF)、糖化血红蛋白(HbAlc)联合检测在非人灵长类糖尿病肾病早期诊断中的应用效果。方法恒河猴5只,小剂量30 mg/kg多次静脉注射链脲佐菌素(STZ),血糖稳定后观察尿微量白蛋白、转铁蛋白(TRF)、β2-微球蛋白、糖化血红蛋白及血脂指标。连续观察13个月。结果动物的高血糖状态持续6个月后开始出现胆固醇(TC)、高密度脂蛋白(HDL-C)代谢异常。甘油三酯(TG)和低密度脂蛋白(LDL-C)9个月开始出现代谢异常。尿mALB与β2-MG呈正相关(F=0.242,0.153,P<0.05),TRF与β2-MG呈正相关(F=0.213,P<0.05),HbAlc与β2-MG呈正相关(F=0.153,0.097,P<0.05)。单因素线性回归分析发现TRF是β2-MG和mALB的相关因子(P<0.05)。结论尿微量白蛋白、尿β2-微球蛋白、转铁蛋白、糖化血红蛋白及血脂联合检测可很好的应用于恒河猴动物模型,对糖尿病模型动物早期监控、诊断、治疗及预后有重要意义。Objective To explore the application value of combined detection of microalbumin, β2-microglobulin, TRF, glyeated hemoglobin and serum lipids in diabetic rhesus macaque models. Methods Streptozotocin in a dose of 30 mg/kg of body weight was intravenously injected to five rhesus macaque for consecutive 3 days. The dynamic changes of u- rine microalbumin, β2-microglobulin, TRF, glyeated hemoglobin were measured for 13 months. Results β2-microglobu- lin and transferrin were significantly higher than those before STZ injection （P 〈 0. 05 ）. The metabolism of TC and HDL-C were abnormal 6 months after STZ injection. Abnormal TG and LDL-C metabolism occurred 9 months after STZ injection. Significantly positive correlations were observed between TRF and β2-MG （ F = 0. 242, 0. 153, P 〈 0. 05 ） , mALB andTRF （F =0. 213, P 〈 0. 05）, as well as HbAlc and β2-MGTRF （ F=0. 153, 0. 097, P 〈 0. 05）. There was a positive correlation between urine mALB and 132-MG （F =0. 242, 0. 153, P 〈 0. 05）, between TRF and β2-MG （F =0. 213, P 〈 0. 05） , and between HbAlc and β2-MG （F = 0. 153, 0. 097, P 〈 0. 05）. Single factor linear regression analysis showed that TRF was a correlation factor of mALB and β2-MG （ P 〈 0. 05）. Conclusions The results show that the combined de- tection of urine microalbumin, β2-microglobulin, glycated hemoglobin and serum lipids can be used in rhesus macaque mod- els of diabetes mellitus, and are useful in their early monitoring, diagnosis, therapy and prognosis.

关 键 词：尿微量白蛋白 尿Β2-微球蛋白 糖化血红蛋白 恒河猴 

分 类 号：Q95-33[生物学—动物学]