微电极阵列芯片技术研究48h房颤犬左、右心耳电生理特性  

Electrophysiological characteristics of the right and left atrial appendages in 48-h atrial fibrillation canine detected by microelectrode arrays chip

作　　者：张玲[1] 孙娟[1] 马嵋[2] 周祁娜[1] 王凌鹏[1] 王欢[1] 侯月梅[1] 

机构地区：[1]新疆医科大学第一附属医院心律失常实验室,乌鲁木齐830054 [2]新疆医科大学第一附属医院医学动物模型研究实验室,乌鲁木齐830054

出　　处：《中国实验动物学报》2012年第6期38-42,I0005,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家国际科技合作专项项目(2011DFA32860);国家自然科学资助基金项目(30960132)

摘　　要：目的应用微电极阵列芯片(microelectrode arrays chip,MEA)技术评价48 h房颤(atrial fibrillation,AF)犬左、右心耳(LAA、RAA)的电生理特性。方法随意来源犬12只,以600次/分起搏右心房建立AF模型,分为48 h AF组(n=6)和对照组(n=6)。造模成功后迅速开胸剪取LAA、RAA,置于盛有台式液的MEA中,分别记录AF组及对照组LAA、RAA场电位(field action potential,FAP)形态、振幅、放电频率及激动传导情况。结果 AF组LAA、RAA组织FAP节律绝对不齐,LAA(185.22±25.62)次/分,较对照组(156.44±8.88)次/分增加15.67%(P<0.01),RAA(102.39±16)次/分,较对照组(156.44±8.88)次/分减慢34.62%(P<0.01)。48 h AF组LAA组织电压(458.33±26.73)μV较对照组(740.55±18.93)μV降低38.11%(P<0.01),RAA(504.83±39.93)μV较对照组(840.56±18.93)μV明显降低(P<0.01),48 h房颤组LAA组织FAP时程(45.28±8.59)ms较对照组(70.77±6.98)ms缩短15 ms(P<0.01)。RAA(61.78±7.1)ms较对照组(75.83±7.63)ms缩短14 ms(P<0.01)。48 h AF组LAA、RAA FAP传导异质性增加。结论应用MEA技术可反映心肌组织片场电位电生理特性,48 h AF后LAA放电频率增加,频率绝对不齐,LAA、RAA电压降低,场电位时程延长。Objective To explore the electrophysiological characteristics of right atrial appendage （RAA） and left atrial appendage （LAA） in 48-h atrial fibrillation （AF） canine detected by microeleetrode array （MEA） chip tech- nique. Methods Twelve adult healthy mongrel canine were randomly divided into two groups：48-h pacing group and con- trol group. The 48-h AF model was established by rapid pacing the right atrium. The RAA and LAA were quickly removed and immersed into Tyrode＇ s solution in MEA chip dish. The morphology, duration, voltage, discharge frequency and con- duction of field action potential were recorded. Results In the AF group, the rhythms of LAA and RAA tissue field poten- tial were irregular. The frequency of LAA （ 185.22 ± 25.62 beats/min） was increased by 15.67% （ 156. 44 ± 8.88 beats/ min） （ P 〈 0. 01 ）, and the frequency of RAA （ 102.39±16 beats/min） was decreased by 34. 62% than that in the control group （156. 44 ± 8.88 beats/min） （P 〈 0. O1 ）. The voltages of LAA （458.33 ± 26.73 ） μV and RAA （504. 83 ±39.93 ）μV were decreased than those of the control group （740. 55 ± 18.93）μV and （840. 56 ±18.93 ）μV, respectively, （P 〈 0. 01 ）. The durations of field potential of LAA （45.28± 8.59） ms and RAA （61.78 ± 7.1 ） ms were significantly shortened than those of the control group （70. 77 ±6. 98）ms （P 〈 0. 01 ）. The electric impulse presented anisotropic alteration. Conclusions MEA is a sensitive, long-lasting and stable technique, and local tissue action potential can be used to assess the electrophysiological characteristics of field potential in animal heart slices using multiple-channel recording mapping sys- tem. The discharge frequency of left atrial appendage is increased and frequency irregularity occurs after 48 h atrial fibrilla- tion, the voltage of field potential is decreased in the atrial appendage, and the field potential duration is prolonged.

关 键 词：心房颤动 微电极阵列 场电位 电生理学 左心耳 右心耳 随意来源犬 

分 类 号：Q95-33[生物学—动物学]