海洛因成瘾模型建立及中脑腹侧被盖区Bax的表达  

Effect of heroin on Bax expression in the brain ventral tegmental area and establishment of a rat model of heroin addiction

作　　者：俞发荣 连秀珍 谢明仁 张红梅 李立新 张琛 李世平 

机构地区：[1]甘肃省证据科学技术研究与应用重点实验室,兰州730070

出　　处：《中国实验动物学报》2012年第6期82-84,I0008,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：甘肃省自然科学基金(编号:甘财教[2010]148号);甘肃省高校学科带头人扶持项目计划基金(编号:甘教字[2011]23号);甘肃省证据科学技术研究与应用重点实验室科技计划项目基金(编号:ZDSYS-Y17)

摘　　要：目的探索海洛因对中脑腹侧被盖区细胞Bax表达的影响。方法肌肉注射海洛因,建立成瘾大鼠模型,用免疫组化方法检测中脑腹侧被盖区细胞Bax的表达。结果连续给大鼠注射海洛因7d后,大鼠出现明显的戒断症状;中脑腹侧被盖区细胞Bax表达阳性细胞比对照组明显增多,与对照组相比差异有显著性(P<0.01)。结论海洛因具有诱导Bax基因表达、损伤脑组织细胞的作用。Objective To study the effects of heroin on Bax expression in the midbrain ventral tegmental area （VTA） of rat. Methods Heroin was intramuscularly injected into rats and a rat model of addiction was generated. Bax expression in the midbrain ventral tegmental area （VTA） was detected by immunohistochemistry. Results The rats had significant withdrawal symptoms after continuous injection of heroin for 7 days. The expression of Bax-positive cells in cere- bellar cortex of rat was significantly increased than that in the control group （ P 〈 0. 01 ）. Conclusion Heroin has effect of inducing Bax gene expression and damage to brain cells.

关 键 词：海洛因 BAX 中脑腹侧被盖区 大鼠 

分 类 号：Q95-33[生物学—动物学]