γ射线照射对椎间盘生物学活性的影响  

Effect of γ irradiation on biological properties of intervertebral disc allografts

作　　者：丁宇[1] 阮狄克[1] 何勍[1] 王超锋[1] 

机构地区：[1]海军总医院骨科,北京100048

出　　处：《脊柱外科杂志》2012年第6期360-365,共6页Journal of Spinal Surgery

基　　金：国家自然科学基金(30730095)

摘　　要：目的探讨γ射线照射处理异体椎间盘的可行性,同时进一步评估去细胞化椎间盘作为自然支架辅助生物学治疗椎间盘退变的性能。方法手术获取6例成熟比格犬的椎间盘,逐级冷冻保存,随机分为对照组及18 kGy、25 kGy、50 kGyγ射线照射组(n=8),处理各组椎间盘后进行细胞活性及生物力学检测并进行对比分析。结果椎间盘细胞活性与照射剂量成负相关,对照组纤维环及髓核细胞活性分别为92.6%、90.7%,18 kGy照射组为76.5%、70.6%,25 kGy照射组为52.7%、46.9%,50 kGy照射组为18.3%、10.1%,各组间差异有统计学意义(P<0.01)。不同组间椎间盘生物力学性能差异均无统计学意义(P>0.05),其中压缩、拉伸轴向力及左/右旋转扭力矩均维持在正常水平。结论优化γ射线照射可以作为异体椎间盘消毒灭菌的手段,但其确切效果有待体内实验进一步验证。去细胞化椎间盘具有较为理想的生物学及机械性能,有望作为自然支架用于椎间盘疾病的相关研究。Objective To explore the feasibility of optimized γ irradiation for management of disc allografts, and to assess the performance of decellularized allograft discs as the natural disc scaffold for biological treatment of disc degeneration. Meth- ods Discs were harvested from 6 beagles and gradually frozen in RPMI-1640 solution, and then they were randomly divided into 4 groups： control group （discs with no irradiation） and 3 irradiating groups （discs irradiated at doses of 18 kGy, 25 kGy and 50 kGy, n = 8 ）. Cell viability and biomechanical examinations were done for each group and the results were analyzed and compared. Results The disc cell viability was negatively correlated with the irradiation dose. The cell viabilities of the annulus fibrosus and nucleus pulposus were 92.6% and 90.7% in control group, 76.5% and 70.6% in the 18 kGy group, 52.7% and 46.9% in the 25 kGy group, and 18.3% and 10. 1% in 50 kGy group, respectively. There were significant differences between different groups （P 〈 0. 01 ）. No significant differences were found for the biomechanieal properties among the 4 groups. The axial forces and torsional torques all maintained the normal level （ P 〉 0.05 ）. Conclusion Optimized irradiation can serve as an effective sterilization method for allograft discs, but the effect needs to be further studied in vivo. As a natural scaffold, decelluarized disc allograft has the ideal physiology and biomechanics properties to serve as natural scaf- fold for research on disc disorders.

关 键 词：椎间盘 骨移植 移植预处理 Γ射线 细胞存活 生物力学 

分 类 号：R318.01[医药卫生—生物医学工程]