人工合成麝香对胚胎干细胞发育毒性的初步研究  

The developmental toxicity assay of synthetic musks using the mouse embryonic stem cells

作　　者：施嘉琛[1,2] 张晶[1,2] 邵兵[1,2] 

机构地区：[1]北京市疾病预防控制中心,北京100013 [2]北京市预防医学研究中心

出　　处：《毒理学杂志》2012年第6期412-414,共3页Journal of Toxicology

基　　金：国家自然科学基金(20877010;20837003)

摘　　要：目的研究常用人工合成麝香吐纳麝香(AHTN)和佳乐麝香(HHCB)对129小鼠胚胎干细胞的发育毒性效应。方法利用MTT法检测两种麝香对胚胎干细胞的毒性效应,结合不同暴露浓度下麝香对胚胎干细胞体外分化为心肌细胞能力的抑制情况,判断受试物的发育毒性。结果 MTT实验表明,AHTN和HHCB对小鼠胚胎干细胞半数毒性浓度IC50分别为34和29μg/ml,而分化抑制实验表明这两种麝香对胚胎干细胞向心肌细胞的半数分化抑制浓度ID50分别为257和153μg/ml。结论人工麝香对胚胎发育具有弱发育毒性,对孕妇和儿童发育的影响需要在相关的发育毒性研究中给予重视。Objective To investigate the potential embryotoxicity of two commonly used musks, tonalide （AHTN） and galaxolide （HHCB）, using the 129 mouse embryonic stem cells. Methods The cytotoxicity assay with MTT method and differentiation assay were used to determinate the potential developmental toxicities of the musks. Results The cytotoxicity assay showed the ICso of AHTN and HHCB were 34 and 29 μg/ml. The differentiation assay showed the IDs0 of AHTN and HHCB were 257 and 153 μg/ml. Conclusion Both AHTN and HHCB were evaluated as weak-embryotoxicity, the toxicological effect of these compounds should be further concerned in future study.

关 键 词：人工麝香 吐纳麝香(AHTN) 佳乐麝香(HHCB) 胚胎干细胞 

分 类 号：R994.6[医药卫生—毒理学]