猪嵴病毒VP1基因的克隆及生物信息学分析  

Cloning and bioinformatics analysis of porcine kobuvirus VP1 protein gene

作　　者：陈蕾[1,2] 朱玲[1,2] 李淞[1,2] 周远成[1,2] 徐志文[1,2] 

机构地区：[1]四川农业大学动物生物技术中心,四川雅安625014 [2]动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《中国兽医科学》2012年第12期1254-1258,共5页Chinese Veterinary Science

基　　金：四川省科技支撑计划项目(2012NZ0001)

摘　　要：为研究猪嵴病毒VP1蛋白的结构与功能,运用RT-PCR技术扩增猪嵴病毒VP1基因,测序并对其进行生物信息学分析。结果显示,成功获得了762bp的猪嵴病毒VP1基因(GenBank登录号为JX294863)。经生物信息学分析,此序列编码253个氨基酸,理论等电点(pI)为4.81,理论分子质量为26 879.2u,不稳定系数为35.93;在同一氨基酸的不同密码子的选择上存在一定的偏向性;最大疏水指数为1.967,最小疏水指数为-2.244;无信号肽和跨膜区;N末端和C末端抗原指数较高,预测抗原表位位于这些区域;蛋白核心结构为8个β折叠片围成一个柱状结构;进化树分析结果显示,JX294863与匈牙利株在一个分支上,两者亲缘关系较近。Porcine kobuvirus VP1 gene was cloned by RT-PCR and sequenced,then bioinformatic analysis were conducted to investigate the structure and function of the porcine kobuvirus VP1 gene.In result,a 762 bp porcine kobuvirus VP1 gene was obtained（GenBank accession number：JX294863）.Bioinformatics analysis showed that the sequence encoded 253 aa.The isoelectric point,molecular weight and instability index of porcine kobuvirus VP1 were 4.81,26 879.2 u and 35.93,respectively.An amino acid had bias of codons choosing.The maximum and the minimum hydrophobicity were 1.967 and-2.244,respectively.The protein had no signal peptide and transmembrane domain.Antigenic index of N-terminal and C-terminal were higher.Antigenic epitope may locate in these regions.Main structure of the VP1 protein was a column structure made up of eight beta-pleated sheet.Evolutionary tree showed that JX294863 was on the same branch with a Hungary strain.The results provided the foundation for further studying biological functions of the gene and for establishing diagnosis method.

关 键 词：猪嵴病毒 VP1基因 克隆 生物信息学分析 

分 类 号：S852.659.6[农业科学—基础兽医学]