羊毛及羊绒制品实时荧光PCR鉴定方法研究  

Real-time PCR Identification of Wool and Cashmere Products

作　　者：陈国培[1] 赖心田[1] 唐复润[1] 林霖[1] 杨友红[1] 董晶泊[1] 杨志敏[1] 杨国武[1] 

机构地区：[1]深圳市计量质量检测研究院

出　　处：《纺织导报》2013年第2期88-91,共4页China Textile Leader

基　　金：广东省质量技术监督局科技项目(2011CZ12)

摘　　要：羊毛及羊绒制品是我国重要的纺织产品。市面上以次充好的掺假现象时常发生,建立客观、稳定、快速、准确的鉴别方法是当前的迫切之需。本文采用分子生物学手段对羊毛及羊绒制品进行鉴定,开发了适用于羊毛及羊绒制品的DNA提取方法,并建立了荧光PCR检测体系,同时进行方法特异性、检出限分析,结果表明本方法特异性好,检出限为10ngDNA(含担体DNA)。采用建立的方法体系对16个样品进行检测,结果表明本方法与传统鉴定方法具有一致性,灵敏度比传统方法更优,具有应用于羊毛及羊绒制品日常检测的可行性。Wool and cashmere are important textile materials in China. Subjected to the limitation of identification methodology, adulteration and false declaration of wool and cashmere products are common on the market. Hence, there is an urgent need to develop a quick, objective, reliable and accurate identification method. In this paper, DNA extraction protocol of wool and cashmere products was developed, common primers and species-specific probes for real-time PCR were designed from mitochondrial 16S ribosomal RNA sequences, and the method specificity, detection limit and sample analysis were conducted in order to assess the sensitivity, stability and specificity of the established method. Sensitivity experiment showed that wool and cashmere can be distinguished from other textile products using the species-specific probes. Sensitivity experiment also demonstrated that the detection limit of the established method is 10ng DNA (carrier DNA contained), and the analysis of sixteen textile sample revealed that the established method is consistent with traditional identification method and much more sensitive than traditional method, proving the feasibility of using the established method in the routine inspection work.

关 键 词：羊毛 羊绒 PCR鉴定方法 DNA提取方法 

分 类 号：TS101.921[轻工技术与工程—纺织工程]