不同植物生长调节剂对“金傲芬”无花果组培的影响  

Effect of Different Plant Growth Regulators on Tissue Culture of Ficus carica L.‘Jin Ao-fen ’

作　　者：陈爽[1] 李金平[1] 马会勤[1] 张文[1] 

机构地区：[1]中国农业大学农学与生物技术学院,果树逆境生理与分子生物学北京市重点实验室,北京100193

出　　处：《北方园艺》2013年第3期109-111,共3页Northern Horticulture

基　　金：北京市都市农业学科群建设资助项目;北京市园林绿化局计划资助项目

摘　　要：以无花果"金傲芬"幼嫩带芽茎段为外植体,MS为基本培养基,采用常规组织培养方法,研究了不同植物生长调节剂对"金傲芬"无花果组织培养的影响。结果表明:2%的NaClO对外植体消毒6.5min能起到较好的消毒效果,污染率为28%;适合"金傲芬"组培的初始培养基为MS+6-BA 2.0mg/L+NAA 0.2mg/L+蔗糖30g/L+琼脂8.0g/L(pH 5.8);壮苗培养基为MS+6-BA 0.5mg/L+NAA 0.2mg/L+GA 0.2mg/L+蔗糖30g/L+琼脂8.0g/L(pH 5.8);生根培养基为MS+IBA 1.0mg/L+蔗糖30g/L+琼脂8.0g/L(pH 5.8)。Taking young stems with buds of ＇Jin Ao-fen＇ Ficus carica Linn as explants, on the basic MS medium,the effects of different plant growth regulators on shoot multiplication were studied. The results showed that the most appropriate time of disinfection of 2 % NaC10 was 6.5 min. The most appropriate medium of bud differentiation was MS＋BA 2.0 mg/L＋NAA 0. 2 rng/L＋sucrose 30 g/L＋agar 8. 0 g/L（pH 5. 8）;the most appropriate medium of making bud strong was MS＋6-BA 0. 5 mg/L＋NAA 0. 2 mg/L＋GA 0. 2 rag/L-k-sucrose 30 g/L＋agar 8. 0 g/L（pH 5.8） ;the most appropriate rooting culture medium was MS＋IBA 1.0 mg/ L＋ sucrose 30 g/L＋agar 8. 0 g/L（pH 5. 8）.

关 键 词：无花果 “金傲芬” 组织培养 植物生长调节剂 

分 类 号：S482.8[农业科学—农药学]