正交试验优选桑寄生中槲皮苷的提取工艺  

Optimization of Extraction Technology of Quercitrin from Taxillus chinensis

作　　者：苏本伟[1,2] 张协君 朱开昕[3] 赵明惠[3] 裴河欢 李静 李永华[2] 

机构地区：[1]钦州市中医药研究所,广西钦州535000 [2]广西中医药大学药学院,南宁530001 [3]钦州市中医院,广西钦州535000

出　　处：《中国实验方剂学杂志》2013年第2期8-10,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173537);广西科技攻关项目(桂科攻11107009-1-12)

摘　　要：目的:优选桑寄生中槲皮苷的提取工艺。方法:以槲皮苷提取率为指标,HPLC测定槲皮苷含量,选取甲醇体积分数、甲醇量、超声时间、浸泡时间为考察因素,通过单因素试验和正交试验优选桑寄生中槲皮苷的提取工艺。结果:各因素对槲皮苷提取率的影响顺序为甲醇体积分数>甲醇用量>超声时间>浸泡时间。优选的提取工艺条件为取桑寄生药材粉末加50倍量75%甲醇浸泡1 h后超声处理45 min,槲皮苷提取率8.64 mg.g-1。结论:该提取方法操作简单、方便,为桑寄生槲皮苷的进一步研究提供试验依据。Objective： To optimize extraction technology of quercitrin in Taxillus chinensis. Method： With yield of quercitrin as index, which was determined by HPLC, the concentration and amount of methanol, ultrasonic time, soaking time were chosen as factors, extraction technology of quercitrin from T. chinensis was optimized by single factor test and orthogonal test. Result： The order of four factors affecting extraction yield was volume fraction of methanol 〉 amount of methanol 〉 ultrasonic time 〉 soaking time. Optimized extraction conditions was as following： took powder of T. chinensis, ultrasonic extracted 45 min after soaked 1 h with 50 times the amount of 75% methanol, extraction yield of quercitrin 8.64 mg .g-1 Conclusion： This optimized technology was simple and convenient, it could provide experimental basis for further research of quercitrin from T. chinensis.

关 键 词：桑寄生 槲皮苷 正交试验 提取工艺 

分 类 号：R283.6[医药卫生—中药学]