三七总皂苷注射液的超滤工艺考察  

Investigation of Total Saponin from Panax notoginseng Injection by Ultrafiltration Technology

作　　者：缪菊连[1] 黄照昌 

机构地区：[1]大理学院药学与化学学院,云南大理671003 [2]大理药业股份有限公司,云南大理671000

出　　处：《中国实验方剂学杂志》2013年第2期35-37,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:探索三七总皂苷注射液的超滤工艺。方法:采用HPLC测定人参皂苷Rg1,Rb1及三七皂苷R1的含量,通过单因素试验考察不同相对分子量超滤膜、药液质量浓度、温度、洗液体积对三七总皂苷注射液超滤工艺的影响。结果:确定的超滤工艺为三七总皂苷水溶液质量浓度100 g.L-1,选用10 kDa超滤膜,超滤温度70℃,洗液体积1 200 mL,人参皂苷Rg1,Rb1及三七皂苷R1各组份透过率均>90%。结论:优选的超滤工艺用于三七总皂苷注射液的纯化是可行的。Objective： To explore uhrafiltration technology of total saponin from Panax notoginseng injection. Method： Contents of ginsenoside Rg1 , RbI and notoginsenoside R1 were determined by HPLC, effect of different factors on uhrafiltration technology were investigated by single factor test, including different molecular weight ultrafiltration membrane, the concentration and temperature of solution, lotion volume. Relust： 10 kDa uhrafiltration membrane was chosen, determined ultrafiltration technology was： the mass concentration of water solution of 100 g -L-1, uhrafiltration temperature 70 ℃ , lotion volume 1 200 mL, transmission of ginsenoside Rg1, Rb1 and notoginsenoside R： were more than 90%. Conclusion： This optimized uhrafjltration technology was feasible for purification of total saponin of P. notoginseng.

关 键 词：三七总皂苷 超滤 高效液相色谱法 

分 类 号：R283.6[医药卫生—中药学]