不同来源和品种化橘红果实中肌醇含量比较  

Content Comparison of Myo-Inositol from Citrus grandis of Different Varieties

作　　者：张旭倩[1] 肖凤霞[1] 邓少东[1] 邓超明[1] 邓韬[1] 林励[1] 

机构地区：[1]广州中医药大学中药学院,广州510006

出　　处：《中国实验方剂学杂志》2013年第2期55-57,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部"十二五"科技支撑计划项目(2011BAI01B02);广州中医药大学科研基金项目(06C80)

摘　　要：目的:考察不同来源和品种化橘红中肌醇的含量,为化橘红质量标准和开发利用提供科学依据。方法:采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法进行测定,色谱柱Rezex RCM-Monosaccharide Ca2+(8%)(Phenomenex,7.8mm×300 mm);流动相为去离子水,流速0.40 mL.min-1,飘移管温度80℃,喷雾管加热状态100%加热状态,雾化气体为空气,压力40 bar;柱温35℃。结果:肌醇在0.25～8.00 g.L-1线性关系良好(r=0.999 9),平均回收率为98.94%,RSD2.19%,样品中其他成分不干扰测定。结论:化橘红不同栽培品种中肌醇含量差别较大,以陆福品种含量最高,在发育过程中可能呈上升趋势,广西市售化橘红与化州基地采收品含量无明显差别。Objective： To determine and compare the content of myo-inositol in Citri Grandis. Method： The content was determined by high performance liquid chromatography coupled with evaporative light-scattering detector （HPLC-ELSD） method. The operation was performed on a Rezex RCM-Monosaccharide Ca2＋ （8%） cloumn （7.8 mm ×300 mm） using deionized water as the mobile phase at the flow rate of 0.4 mL -min-1. The temperature of drift tube was set at 80 ~C , the tube was set at heating 100% and the column temperature at 35 ℃ , thee gas pressure was 40 bar. Result： The calibration curve of inositol displayed ideal linearity over the range of 0.25-8.00 g-L-i （r = 0. 999 9）, and the average recovery was 98.94% with RSD of 2. 19%. The other ingredients in the sample showed no interference. Conclusion ： The content of myo-inositol was different due to the different sample-variety of C. grandis and the sample from Lufu was the highest. And it would show a pattern of increase after pollination. But this study didnt show variations for samples from different region.

关 键 词：肌醇 化橘红 高效液相色谱-蒸发光散射 含量测定 

分 类 号：R284.1[医药卫生—中药学]