巴豆生物碱部位HPLC特征指纹图谱研究  

Study on HPLC Fingerprint of Alkaloids in Croton tiglium

作　　者：金锋[1] 任玉珍[2] 陈彦琳[2] 杜杰[2] 周林[2] 白宗利[2] 梁焕[2] 张振凌[1] 

机构地区：[1]河南中医学院,郑州450008 [2]中国药材公司,北京102600

出　　处：《中国实验方剂学杂志》2013年第2期90-93,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2011年中医药行业科研专项(201107008);中药过程控制技术国家工程实验

摘　　要：目的:建立巴豆生物碱部位的高效液相指纹图谱。方法:色谱柱Waters Symmetry C18(4.6 mm×250 mm,5μm),甲醇-0.2%乙酸水流动相系统梯度洗脱,流速0.8 mL.min-1,检测波长292 nm,温度28℃。结果:对10批巴豆药材进行了测定,建立了巴豆生物碱部位的指纹图谱,共有13个共有峰,指认了两种主要生物碱巴豆苷和木兰花碱。结论:该方法可为巴豆的研究和质量控制提供依据。Objective ： To study chromatography fingerprints of the alkaloids in Croton tiglium by HPLC. Method： Waters Symmetry C18 column （4.6 mm ×250mm, 5μm） was used with methanol-0.2% acetic acid of water gradient elution, at flow-rate of 0. 8 mL ·min-1, detection wavelength at 292 nm; column temperature was at 25℃. Result： HPLC was used to establish fingerprint chromatograph of methanol extraction from thirteen batches of C. tiglium, which had been harvested from the same area at the same time. Crontonoside and magnoflorine in the fingerprint were identified. The result showed that peaks were common. Conclusion： The method could be used for the research and the quality control of C. tiglium.

关 键 词：巴豆 生物碱 高效液相 指纹色谱 

分 类 号：R284.1[医药卫生—中药学]