葛根芩连汤中14种药效组分的HPLC分析  

Analysis of 14 Active Compositions Alignment in Gengen Qinlian Decoction by HPLC

作　　者：毛莹[1,2] 张贵君[1] 刘晶晶[1] 董文茜[1] 张雅楠[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]大连大学医学院,辽宁大连116622

出　　处：《中国实验方剂学杂志》2013年第2期108-113,共6页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:分析测定葛根芩连汤中的14种药效组分,为建立其与临床疗效对应的质量标准提供技术和思路。方法:按照传统经方制备葛根芩连汤,采用高效液相色谱法(HPLC)对其药效组分进行测定。结果:葛根芩连汤中的14种药效组分,即葛根素、大豆苷、大豆苷元、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素、盐酸小檗碱、盐酸巴马汀、盐酸药根碱、盐酸表小檗碱、盐酸黄连碱、甘草酸铵、甘草苷的含量分别为1.673 7%,0.153 3%,0.047 3%,0.448 0%,0.091 6%,0.012 3%,0.006 7%,0.095 5%,0.034 3%,0.043 8%,0.036 0%,0.048 3%,0.110 3%,0.158 0%。结论:所采用的HPLC法快速、简便,所测得的葛根芩连汤中的药效组分可作为其质量控制的科学指标。Objective： To determine 14 active compositions alignment （ACA） in Gengen Qinlian Decoction so as to provide technology and ideas for the establishment of quality standard in accordance with its therapeutic. Method： Gengen Qinlian Decoction was prepared by traditional method, the ACA were determined by high performance liquid chromatography （HPLC）. Result： The 14 ACA in Gengen Qinlian Decoction were puerarin, daidzin, daidzein, baicalin, wogonoside, baicalein, wogonin, berberine hydrochloride, palmatine hydrochloride, jatrorrhizine hydrochloride, epiberberine hydrochloride, coptisine hydrochloride, ammonium glycyrrhizinate, liquiritin. The content of ACA （%） were 1. 673 7%, 0. 153 3%, 0.047 3%, 0.448 0%, 0.091 6% , 0.012 3% , 0.006 7% , 0.095 5% , 0.034 3% , 0.043 8% , 0.036 0% , 0.048 3% , 0. 110 3% , 0. 158 0%. Conclusion： The HPLC method used is rapid and simple, and the ACA in Gengen Qinlian Decoction can be used as the scientific indicator of quality control.

关 键 词：葛根芩连汤 药效组分 HPLC 

分 类 号：R284.1[医药卫生—中药学]