葛根素对6-羟多巴胺致帕金森病模型大鼠黑质组织神经细胞的保护作用  

Protective Effect of Puerarin on Nerve Cells in Substantia Nigra of Parkinson's Disease Rats Induced by 6-hydroxydopamine

作　　者：黎荣[1] 徐灵源[2] 梁韬[3] 张士军[3] 李勇文[1] 段小群[1] 

机构地区：[1]桂林医学院,广西桂林541004 [2]右江民族医学院附属医院药剂科,广西百色533000 [3]广西医科大学,南宁530021

出　　处：《中国实验方剂学杂志》2013年第2期247-250,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究葛根素对6-羟多巴胺(6-OHDA)致帕金森病(PD)模型大鼠黑质组织神经细胞的保护作用。方法:建立帕金森病大鼠模型,随机分成5组:模型组、左旋多巴阳性组及葛根素低、中、高剂量组。ig给予葛根素20,40,80 mg.kg-1,阳性组给予左旋多巴40 mg.kg-1,连续灌胃30 d。检测黑质组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量,进行黑质神经细胞常规染色观察,Western blot法检测黑质组织内诱导型一氧化氮合酶(iNOS)、环-磷酸腺苷(cAMP)反应元件结合蛋白(CREB)蛋白表达。结果:葛根素能缓解帕金森模型大鼠病情。与模型组比较,葛根素有效增加帕金森病大鼠黑质组织中GSH-Px,SOD活性,同时降低MDA含量(P<0.01)。下调黑质组织中iNOS蛋白水平,并上调CREB蛋白表达(P<0.01)。结论:葛根素对6-羟基多巴胺所致PD大鼠黑质组织神经细胞具有保护作用,机制可能与其抑制过氧化应激作用以及调节内源性iNOS、CREB蛋白的表达有关。Objective： To investigate the effect of puerarin on parkinson＇as rats model induced by 6- hydroxydopamine （6-OHDA）. Method： Parkinson＇s rats model was established, the rats were randomly divided into 5 groups ： model group, L-dopa group （40 mg ·kg- 1 ） , low-, medium-and high-dosage groups of puerarin （20, 40, 80 mg .kg-1）. The drugs were intragastrically perfused to rats daily for 30 consecutive days. The activity of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） as well as the contents of MDA in substantia nigra tissue were tested by biochemical method ; the pathological change of nerve cells in substantia nigra was observed by HE staining; then the expressions of induced nitric oxide synthase （ iNOS）, cyclic adenosine monophosphate （cAMP） -response-element-binding-protein （CREB） in substantia nigra were using Western blot analysis. Result： The condition of Parkinson＇s rats model was alleviated via puerarin treatment. Compared to model control group, puerarin significantly elevated the activities of GSH-Px and SOD in substantia nigra tissue of parkinson＇as rats, while the MDA contents was reduced （P 〈 0.01 ） ; and down-regulated the iNOS protein level in substantia nigra tissue, while the expression of CREB was increased （P 〈 0.01 ）. Conclusion： The findings indicate that puerarin has protective effect on the 6-OHDA-induced PD rats the mechanism may be associated with restraining the oxidative stress and regulations of endogenous iNOS, CREB expressions.

关 键 词：葛根素 6-羟多巴胺 帕金森病 过氧化应激 保护作用 

分 类 号：R285.5[医药卫生—中药学]