狼毒多糖对S180荷瘤鼠免疫功能的调控作用  

Euphorbia Fischeriana Plyosaccharide Regulates Immune Function in S180 Tumor-bearing Mice

作　　者：崔立然[1,2] 徐浩[3] 刘吉成[3] 孙永旭[3] 蒋丽艳[3] 

机构地区：[1]齐齐哈尔医学院第一附属医院,黑龙江齐齐哈尔161041 [2]黑龙江中医药大学,哈尔滨150040 [3]齐齐哈尔医学院,黑龙江齐齐哈尔161006

出　　处：《中国实验方剂学杂志》2013年第2期255-258,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(30973902)

摘　　要：目的:观察狼毒多糖(EFP-AW1)对S180荷瘤鼠免疫逃逸功能的影响。方法:将50只雄性ICR小鼠随机分为空白对照组、模型组、狼毒多糖100 mg.kg-1组、环磷酰胺100 mg.kg-1组(CTX)、联合给药组(EFP-AW1+CTX)。分组给药10 d后,通过噻唑蓝(MTT)、酶联免疫吸附实验(ELISA)、流式细胞术(FCM)之方法,检测荷瘤鼠脾淋巴细胞增殖情况、转化生长因子β1(TGF-β1)、T淋巴细胞亚群(CD4+T,CD8+T)表达。结果:狼毒多糖可增加刀豆蛋白A(ConA)/脂多糖(LPS)诱导的荷瘤鼠脾淋巴细胞增殖能力(P<0.05),降低血清中TGF-β1的分泌(P<0.05),增加CD8+T细胞百分含量(P<0.05)。结论:ESP-AW1可通过增强荷瘤鼠抗肿瘤免疫应答功能,抑制肿瘤细胞在体内转移和复发。Objective： To observe euphorbia fischeriana plyosaccharide （EFP-AW1） regulate the effect of immunologic function in S180 tumor-bearing mice. Method： Fifty male institute of lancer research （ICR） mice were randomly divided into 5 groups： control group, model group, EFP-AW1 group, CTX group, EFP-AW1 and CTX group. The mice were grouped and treated ,for 10 days by EFP-AW1. The splenocyte proliferation was measured by MTT in order to assess the effects on the immune activity by EFP-AW1; transforming growth factor （TGF-/31） determination by enzyme linked immunosorbent assay （ELISA） ; CD4 ＋T, CD8＋T tumor detected by flow cytometry in peripheral blood. Result： EFP-AW1 could significantly enhance ConA/LPS-induced T lymphocyte proliferation, and reduce TGF-β1 secretion, and increase the number of CD8 ＋T lymphocytes in peripheral blood. Conclusion： EFP-AW1 can enhance immune response function in S180 tumor-bearing mice, inhibit tumor cell in vivo metastasis and recurrence.

关 键 词：狼毒多糖 免疫逃逸 S180肉瘤 淋巴细胞 T细胞亚群 

分 类 号：R285.5[医药卫生—中药学]