参苓白术散对体外艰难梭菌生长的影响  

Influences of Shenglin Baizhu Powder on in vitro Growth of Clostridium difficile

作　　者：舒青龙[1] 徐刚[1] 叶荷平[1] 章喜林[1] 封勇[1] 魏娜[1] 

机构地区：[1]江西中医学院基础医学院,南昌330004

出　　处：《中国实验方剂学杂志》2013年第2期258-262,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81001680);江西省自然科学基金项目(20122BAB205073)

摘　　要：目的:探讨参苓白术散对抗生素相关性腹泻(AAD)主要致病菌艰难梭菌的体外抑菌作用。方法:按正常培养组(脑心浸液培养基培养)和加药培养组(脑心浸液培养基中加入不同稀释梯度的参苓白术散水煎剂,加药体积分别为体积比为:10-1,10-2,10-3,10-4),应用连续采样和分光光度法,测定4株艰难梭菌((NC-1,NC-2,SH182,和SH186,其中2株为A+B+,2株为A-B+)的体外生长曲线。结果:加药(参苓白术散水煎剂)体积比为:10-1,10-2,10-3,10-4的培养基对艰难梭菌NC-1,以及加药体各为10-1的参苓白术散水煎剂对艰难梭菌NC-2有明显的抑制作用(P<0.05),不同稀释度的加药培养基对4种不同的艰难梭菌均有一定的影响,艰难梭菌最大生长细菌菌落均有一定程度的减少。结论:参苓白术散对AAD病主要致病菌艰难梭菌在体外具一定的抑制作用。Objective： To investigate the in vitro bacteriostatic action of Shenglin Baizhu powder （SBP） on Clostridium difficile, a major pathogen of antibiotic associated diarrhea （AAD）. Method ： The normal cuhure group （cultured on brain heart infusion） and cultured on adding medicine culture group （cultured on brain heart infusion adding a series dilution of with V/V： 1 × 10-1, 10-2, 10 -3, and 10 -4） were used to test the influences of SBP on in vitro growths of four clinic C. difficile isolates （NC-1, NC-2, SH182 ana SH186） including two A ~ B ~ stains and two AB ~ stains respectively, using continual exampling and spectrophotometry methods. Result： The modified brain heart infusion medium adding SBP decoction of whole dilution （V/V： 10-1, 10-2, 10-3 and 10-4） and of the 10-1dilution significantly inhibited the growths of C. difficile NC-1 and C. difficile NC-2 respectively （P 〈 0.05 ） , while modified BHI medium adding other series dilution SBP decoctions exhibited slight influences on all selective C. difficile strains by decreasing the bacterial CFU ·mL-1 （colony forming units per milliliter） maximum. Conclusion： SBP has some bacteriostatic action to influence the growth of AAD pathogens.

关 键 词：艰难梭菌 参苓白术散 抗生素相关性腹泻 抑菌作用 

分 类 号：R285.5[医药卫生—中药学]