黔岭淫羊藿总黄酮类成分对hFOB1.19人SV40转染成骨细胞活性的影响  

Influence of Total Flavones of Epimedium leptorrhizum Stearn on hFOB1.19 Osteoblast Activity

作　　者：刘婷[1] 曹春雨[1] 郝然[1] 回连强[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2013年第2期267-270,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技重大专项课题重大新药创制课题(2009ZX09103-426)

摘　　要：目的:观察黔岭淫羊藿总黄酮类成分(YYH-C)对hFOB1.19人SV40转染成骨细胞活性的影响。方法:培养基连续培养hFOB1.19人SV40转染成骨细胞,采用MTT法测定细胞增殖率,全自动生化分析仪测定碱性磷酸酶(ALP)分泌水平,观察50,25,12.5,6.25,3.13,1.56,0.78,0.39,0.20 mg.L-1YYH-C与细胞培养72 h及连续多次给药与细胞培养1,4,7,10d时对细胞活性的影响。结果:0.78～50 mg.L-1的YYC-C与细胞共培养72 h,能明显促进细胞增殖,对细胞的最大增殖率达132.32%,0.78 mg.L-1剂量组尚能明显促进ALP的分泌;YYH-C连续给药1～4 d对hFOB1.19人SV40转染成骨细胞未见明显的增殖作用和明显的促进细胞分泌ALP的作用;自给药7 d开始,YYH-C不同质量浓度组显示出不同程度的促进细胞增殖的作用,YYH-C 0.78～25.0 mg.L-1呈现明显的量-效关系,随着质量浓度进一步的增大,对细胞的增殖作用不再增加,药效至少持续至给药10 d;12.5,25.0,50.0 mg.L-1在给药7 d,25.0,50.0 mg.L-1在给药10 d均能明显促进细胞分泌ALP。结论:YYH-C能明显促进hFOB1.19人SV40转染成骨细胞的增殖,且能明显促进细胞分泌ALP,提示YYH-C对成骨细胞活性的促进作用可能是其防治骨质疏松症的途径之一。Objective： To observe the influence of total flavones of Epimedium leptorrhizum Steam on hFOB1. 19 osteoblast activity. Method： The hFOB1.19 osteoblast was treated with 50, 25, 12.5, 6.25, 3.13, 1.56, 0. 78, 0. 39, 0.20 mg ·L-1 total flavones of E. leptorrhizum Stearn, and then detected proliferation rate by MTT and alkaline phosphate （ALP） secretion by automatic biochemical analyzer after 72 h （single administration） and 1, 4, 7, 10 d （continuous administration）. Result： 0.78-50 mg .L-1 total flavones of E. leptorrhizum Stearn can promote proliferation of hFOB1. 19 osteoblast by single administration after 72 h. The max proliferation rate is 132.32%. 0.78 mg .L-1 total flavones can also promote the ALP secretion obviously. Total flavones of E. leptorrhizum Stearn has no influence on hFOB1. 19 osteoblast by continuous administration at 14 d, but shows a different degree of promoting effect after 7 d. From 0.78 to 25.0 mg ·L-1, the effect presents an obvious dose relationship. With the further increase of drug concentration, the cell proliferation no longer increasing, and the effect lasted at least until 10 days. 12.5, 25.0, 50.0 mg ·L-1 total flavones of E. leptorrhizum Stearn at the 7 d and 25.0, 50.0 mg ·L-1 total flavones at the 10 d can promote ALP secretion significantly. Conclusion： The total flavones of E. leptorrhizum Stearn can promote hFOB1. 19 osteoblast proliferation and ALP secretion. It shows that the promotion of osteoblast activity by total flavones of E. leptorrhizum Stearn maybe a pathway of prevention and treatment of osteoporosis.

关 键 词：黔岭淫羊藿总黄酮类(YYH-C) hFOB1 19人SV40转染成骨细胞 骨质疏松症 

分 类 号：R285.5[医药卫生—中药学]