RP-HPLC法测定左归丸中梓醇的含量  

Content Determination of Catalpol in Zuogui Pills by HPLC

作　　者：孙琳林[1] 任岩海[2] 陈海玲[3] 卢林[1] 刘艳秋[1] 

机构地区：[1]牡丹江医学院红旗医院中西医结合科,黑龙江牡丹江157011 [2]牡丹江医学院红旗医院脑外科,黑龙江牡丹江157011 [3]杭州市中医院老年病科,杭州310007

出　　处：《中国药房》2013年第4期374-375,共2页China Pharmacy

基　　金：黑龙江省中医药科研项目(No.ZHY10-W70);黑龙江省卫生厅科研课题(No.2011-299);杭州市科技发展计划项目(No.20100633B22)

摘　　要：目的:建立测定左归丸中梓醇含量的方法。方法:采用反相高效液相色谱法。色谱柱为EclipseXDB-C18柱,流动相为乙腈-水(0.7:99.3,V/V),流速为1.0ml/min,柱温为25℃,检测波长为210nm,进样量为5μl。结果:梓醇检测质量浓度在0.0067～0.2160mg/ml范围内与峰面积积分值呈良好的线性关系(r=0.9996);平均回收率为98.5%,RSD=1.72%(n=5)。结论:该方法快速、简便,结果准确、可靠,可以用于左归丸中梓醇的含量测定。OBJECTIVE：To develop a method for the content determination of catalpol in Zuogui pills.METHODS：RP-HPLC system was used.The determination was performed on Eclipse XDB-C 18 column with acetonitrile-water（0.7：99.3,V/V） as mobile phase at the flow rate of 1.0 ml/min.Column temperature was 25 ℃.The detection wavelength was set at 210 nm and injection volume was 5 μl.RESULTS：The linear range of catalpol was 0.006 7-0.216 0 mg/ml（r=0.9996）with an average recoveries of 98.5%（RSD=1.72%,n=5）.CONCLUSION：The method is rapid,simple,accurate and reliable.It can be used for the content determination of catalpol in Zuogui pills.

关 键 词：左归丸 梓醇 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]