不同浓度七氟醚后处理对心肌缺血-再灌注损伤的保护作用  

Cardioprotection effect of different concentration sevoflurance postconditioning on isolated rat heart with myocardial ischemia-reperfusion injury

作　　者：陈洪涛[1] 李宝金[1] 雷春亮[1] 杨承祥[2] 

机构地区：[1]广州市第八人民医院麻醉科,510060 [2]佛山市第一人民医院麻醉科

出　　处：《临床麻醉学杂志》2012年第12期1216-1219,共4页Journal of Clinical Anesthesiology

摘　　要：目的探讨不同浓度七氟醚后处理对大鼠离体心脏缺血-再灌注损伤的保护作用。方法 SD雄性大鼠90只,随机分为六组(n=15):N组灌注150min,其余各组灌注30min,然后全心缺血30min,再灌注90min;I、S1、S2、S3、S4组在再灌注开始时分别给予含0%、1%、2%、3%、4%七氟醚的K-H液灌注5min。记录左室舒张末压(LVEDP)、左室发展压(LVDP)。再灌注后测定心肌梗死面积,评估线粒体损伤程度,检测线粒体和胞质细胞色素C水平。结果与N组相比,I组和S1、S2、S3、S4组LVEDP、胞质细胞色素C水平明显升高,LVDP、线粒体细胞色素C水平明显降低(P<0.05)。与I组和S1组相比,S2、S3、S4组LVEDP、胞质细胞色素C水平明显降低,LVDP、线粒体细胞色素C水平明显升高(P<0.05)。再灌注后I组和S1组的梗死面积明显大于S2、S3、S4组(P<0.05)。结论 2%～4%七氟醚后处理对大鼠离体心脏再灌注损伤有明显的保护作用,可能与减轻线粒体损伤,抑制细胞色素C释放有关。Objective To investigate the cardioprotection effect of different concentration sevoflurance postconditioning on isolated rat heart with myocardial ischemia-reperfusion injury. Methods Ninety SD rats＇ were randomly divided into 6 groups （n = 15）： group N was perfused for 150 rain, while the other five groups were perfused for 30 min, ischemia for 30 min, and reperfused for 90 miru At the beginning of the reperfusion, these five groups were reperfused with K-H fluid containing 0% （group I）, 1%（group S1）, 2%（group S2）, 3% （group Sa）, and 40% （ group S4） sevoflurane for 5 min, respectively. Left ventricular diastolic pressure （LVEDP）, left ventricular developed pressure （LVDP） were recorded. After the reperfusion, acute infarct size, mitochondrial damage, and cytoplasm and mitochondrial levels of cytochrome C were evaluated. Results Compared with group N, LVEDP and cytoplasma cytochrome C levels increased and LVDP, mitochondria cytochrome C levels decreased in groups I, S1, S2, S3, and 84 （P〈0.05）. Compared with groups I and S1, LVEDP and cytoplasma cytochrome C levels decreased and LVDP, mitochondria cytochrome C levels increased in groups S2, S3, and S4 （P%0.05）. After the reperfusion, infarct sizes were higher in groups S1 and I than in groups S2, Sa, 84 （P〈0.05）. Conclusion Sevoflurance at the concentrations of 2 %-4 % protects against myocardial ischemia-reperfusion injury of isolated rat heart, which may be attributed to the decreased mitochondrial damage and cytochrome C release.

关 键 词：七氟醚 缺血一再灌注损伤 线粒体 细胞色素C 

分 类 号：R614[医药卫生—麻醉学]