孕早期氯胺酮麻醉对子代大鼠海马hippyragraninmRNA表达的影响  

Effect of ketamine anesthesia in early pregnancy on expression of hippyragranin mRNA in hippocampus in offsprings of rats

作　　者：李兵达[1] 罗佛全[1] 赵为禄[1] 汤洋[1] 张勤[1] 胡艳[1] 

机构地区：[1]南昌大学第一附属医院麻醉科,330006

出　　处：《中华麻醉学杂志》2012年第11期1334-1336,共3页Chinese Journal of Anesthesiology

基　　金：基金项目：国家自然科学基金（81060093）

摘　　要：目的探讨孕早期氯胺酮麻醉对子代大鼠认知功能及海马hippyragranin（HGN）mRNA表达的影响。方法清洁级孕5～7d的sD大鼠120只，月龄7周，体重270～300g，采用随机数表法，将其随机分为4组：氯胺酮麻醉2h组（K1）、4h组（K2）、8h组（K3）和对照组（C组），每组30只。K1组～k组孕鼠经尾静脉注射氯胺酮20mg／kg，翻正反射消失后，以130mg·kg^-1·h^-1的速率静脉输注，维持时间分别为2、4和8h，C组给予等容量生理盐水。子鼠出生30d时进行Moms水迷宫实验。于Morris水迷宫实验结束后第2天，处死子鼠，采用RealTimePCR法测定海马HGNmRNA的表达水平。结果与C组比较，K1组～k组子鼠逃避潜伏期延长，穿越原平台次数和原平台所在象限停留时间缩短，海马HGNmRNA表达水平上调（P〈0．05）；K1组～K3组间子鼠逃避潜伏期、穿越原平台次数和原平台所在象限停留时间差异无统计学意义（P〉0．05）。与K1组比较，K3组子鼠海马HGNmRNA表达水平上调（P〈0．05），K2组差异无统计学意义（P〉0．05）；K2组和K3组子鼠海马HGNmRNA表达水平差异无统计学意义（P〉0．05）。结论孕早期氯胺酮麻醉对子代大鼠学习记忆功能损害的机制可能与其上调子代海马HGNmRNA的表达有关。Objective To investigate the effect of ketamine anesthesia in early pregnancy on expression of hippyragranin （HGN） mRNA in hippocampus in offsprings of rats. Methods One hundred and twenty pregnant Sprague-Dawley rats at 5-7 days of gestation, aged 7 weeks, weighing 270-300 g, were randomly divided into 4 groups （ n = 30 each） ： 2 h anesthesia with ketamine group （ group K1 ）, 4 h anesthesia with ketamine group （group K2 ）, 8 h anesthesia with ketamine group （group K3 ） and control group （group C）. Ketamine 20 mg/kg was injected via the caudal vein, followed by continuous intravenous infusion at 130 mg ·kg^- 1 . h^- 1 after loss of righting reflex, and the infusion lasted for 2, g and 8 h in groups K~ - K3, respectively. The equal volume of nor- mal saline was given instead of ketamine in group C. The learning ability and memory of the offsprings were tested by Morris water maze test on postnatal day 30. The offsprings were sacrificed on 2nd day after the end of Morris water maze test, their brains were removed and hippocampi were isolated for determination of the expression of HGN mRNA by RT-PCR.Results Compared with group C, the escape latency was significantly prolonged, the frequency of crossing the original platform was decreased, the time of staying at the original platform quadrant was shortened, and the expression of HGN mRNA was up-regulated in groups KI -K3 （ P 〈 0.05 ） . There was no signifi- cant difference in the escape latency, frequency of crossing the original platform and time of staying at the originalplatform quadrant among groups K1- K3 （ P 〉 0.05）. The expression of HGN mRNA was significantly higher in group K3 that in group K1 （P 〈 0.05） . There was no significant difference in the expression of HGN mRNA be- tween group K2 and group K1 （ P 〉 0.05）. Conclusion The mechanism by which ketamine anesthesia in the early pregnancy induces impairment of learning ability and memory of the offsprings is related to up-regulation of HGN mRNA expression

关 键 词：氯胺酮 麻醉 神经组织蛋白质类 妊娠初期 认知障碍 

分 类 号：R965[医药卫生—药理学]