饱和氢盐水对老龄小鼠术后认知功能障碍的影响  

Effects of hydrogen-rich saline on postoperative cognitive dysfunction in aged mice

作　　者：陶苏蔚[1] 信玉昌[1] 周迅[1] 

机构地区：[1]苏州大学附属第二医院麻醉科,215004

出　　处：《中华麻醉学杂志》2012年第11期1344-1346,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价饱和氢盐水对老龄小鼠术后认知功能障碍（POCD）的影响。方法C57BU6雄性小鼠72只，18月龄，体重45～55g，采用随机数字表法，将小鼠随机分为4组（n=18）：对照组（C组）、饱和氢盐水组（H组）、POCD组和POCD＋饱和氢盐水组（PH组）。POCD组采用开放性胫骨骨折手术制备小鼠POCD模型，H组和PH组术前7d开始，每天早晚2次腹腔注射饱和氢盐水10ml／kg。于术后1、3和7d时各组随机取6只小鼠行水迷宫实验测定小鼠认知功能，随后处死取海马，采用ELISA法测定TNF-α和IL-18含量，检测NF-κB活性。结果与c组比较，POCD组术后3和7d时逃避潜伏期延长，目标象限停留时间缩短，穿越平台次数减少，术后1、3和7d时海马TNF—α、IL-1β含量及NF—xB活性升高（P〈0．05），H组各指标差异无统计学意义（P〉0．05）；与POCD组比较，PH组术后3和7d时逃避潜伏期缩短，目标象限停留时间延长，穿越平台次数增加，术后1、3和7d时海马TNF—α、IL-1β含量及NF-κB活性降低（P〈0．05）。结论饱和氢盐水可减轻小鼠术后认知功能障碍，其机制与抑制海马NK—κB活性，降低炎性反应有关。Objective To investigate the effects of hydrogen-rich saline on postoperative cognitive dys- function （POCD） in aged mice and the possible mechanism. Methods Seventy-two male C57BL/6 mice, aged 18 months, weighing 45-55 g, were randomly divided into 4 groups （ n = 18 each） ： control group （group C）, hydro- gen-rich saline group （group H）, POCD group and POCD ＋ hydrogen-rich saline group （group PH）. POCD was induced by open tibial fracture. Hydrogen-rich saline 10 ml/kg was injected intraperitoneally twice a day for 7 days before surgery. Six mice were chosen at day 1, 3 and 7 after surgery and cognitive function was assessed by Morris water maze test. The mice were then sacrificed and hippoeampus was isolated for determination of tumor necrosis factor-α （TNF-α） and interleukin- 1β （ IL- 1 β） contents （ by ELISA） and nuclear factor-κB （NF-κB） activity. Re- suits Compared with group C, the escape latency was significantly prolonged, the time of staying at the original platform quadrant was shortened, the frequency of crossing the original platform was decreased at 3 and 7 days after surgery, and the contents of NF-α and IL-1β and NF-κB activity were increased during 1,3 and 7 days after surgery in group POCD （P 〈 0.05）, and no significant change was found in the parameters mentioned above in group H （P 〉 0.05）. Compared with group POCD, the escape latency was significantly shortened, the time of staying at the original platform quadrant was prolonged, the frequency of crossing the original platform was increased at 3 and 7 days after surgery, and the contents of NF-α and IL-1β and NF-κB activity were decreased at 1, 3 and 7 days af- ter surgery. Conclusion Hydrogen-rich saline can alleviate POCD via inhibiting NK-κB activity in hippocampus and reducing intlammatory response.

关 键 词：氢 认知障碍 NF-ΚB 

分 类 号：R614[医药卫生—麻醉学]